Protein Variants | Comment | Organism |
---|---|---|
additional information | reteplase is a bacterially expressed TPA deletion mutant comprising residues 176-527 of wild-type TPA. Plasminogen is activated to plasmin by reteplase, and then reteplase is completely cleaved by plasmin into a two-chain form, which is composed of Kringle-2 and P domain linked by a disulfide bond | Rattus norvegicus |
S478A | non-proteolytic TPA full-length mutant | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
plasminogen + H2O | Rattus norvegicus | - |
plasmin + ? | - |
? | |
plasminogen + H2O | Rattus norvegicus Sprague-Dawley | - |
plasmin + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Rattus norvegicus Sprague-Dawley | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
casein + H2O | endogenous TPA activities in the ipsilateral ischemic hemisphere are measured using casein-zymography | Rattus norvegicus | ? | - |
? | |
casein + H2O | endogenous TPA activities in the ipsilateral ischemic hemisphere are measured using casein-zymography | Rattus norvegicus Sprague-Dawley | ? | - |
? | |
plasminogen + H2O | - |
Rattus norvegicus | plasmin + ? | - |
? | |
plasminogen + H2O | - |
Rattus norvegicus Sprague-Dawley | plasmin + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Alteplase | - |
Rattus norvegicus |
More | alteplase is the full-length recombinant human TPA, while reteplase (K2P) is the domain deletion mutant comprising only the Kringle-2 domain and protease domain of TPA | Rattus norvegicus |
Tissue plasminogen activator | - |
Rattus norvegicus |
tPA | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Rattus norvegicus |
General Information | Comment | Organism |
---|---|---|
additional information | alteplase is the full-length recombinant human TPA, while reteplase, K2P, is the domain deletion mutant comprising only the Kringle-2 domain and protease domain of TPA. Reteplase shows a better protective effect than alteplase, suggesting that F, P, or K1 domain in TPA diminishes the brain-protective effect | Rattus norvegicus |
physiological function | the Kringle-2 domain, residues 176-262 of TPA, is the essential structure required for the brain-protective activity of TPA, employing the rat middle cerebral artery occlusion, MCAO, model. The Kringle-2 domain of tissue plasminogen activator significantly reduces mortality and brain infarction in middle cerebral artery occlusion rats. Tissue plasminogen activator shows brainprotective activity within the first 15 min after cerebral ischemia in rats | Rattus norvegicus |