Cloned (Comment) | Organism |
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the gene encoding full-length human t-PA is cloned into pPICZalphaA expression vector downstream of alcohol oxidase promoter and alpha-mating signal sequence from Saccharomyces cerevisiae and flush with the kex2 cleavage site to express the protein with a native N-terminus. Pichia pastoris strain GS115 is transformed with this cassette, and methanol utilizing (mut+) transformants are selected for production and secretion of functional human t-PA into culture media. Subcloning in Escherichia coli strain Top10 F' | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
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Subunits | Comment | Organism |
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More | t-PA is a serine-protease enzyme containing 527 amino acid residues in five structural domains. The correct folding of t-PA requires the correct pairing of 17 disulfide bridges in the molecule | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
Tissue plasminogen activator | - |
Homo sapiens |
tPA | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
additional information | t-PA is a serine-protease enzyme containing 527 amino acid residues in five structural domains | Homo sapiens |
physiological function | tissue plasminogen activator, t-PA, plays a pivotal role in the treatment of acute myocardial infarction, ischemic stroke, and deep vein thrombosis | Homo sapiens |