Literature summary for 3.4.21.68 extracted from

Thelwell, C.; Longstaff, C.
The regulation by fibrinogen and fibrin of tissue plasminogen activator kinetics and inhibition by plasminogen activator inhibitor 1 (2007), J. Thromb. Haemost., 5, 804-811.

Search for more literature for 3.4.21.68

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in SF9 cells using the Bac-to-Bac baculovirus system	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
plasminogen activator inhibitor-1 (PAI-1)	sctPA is more susceptible to PAI-1 in buffer solution and in the presence of fibrinogen. In the presence of fibrin there is no difference between single-chain enzyme tPA (sctPA) and two chain tPA (tctPA)	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.000086	-	D-Ile-Pro-Arg-p-nitroanilide	value for sctPA, in the presence of stimulater Fibrin P (treated with plasmin-Sepharose)	Homo sapiens
0.000087	-	D-Ile-Pro-Arg-p-nitroanilide	value for sctPA, in the presence of stimulater Fibrin normal	Homo sapiens
0.000109	-	D-Ile-Pro-Arg-p-nitroanilide	value for tctPA, in the presence of stimulater Fibrin N	Homo sapiens
0.000119	-	D-Ile-Pro-Arg-p-nitroanilide	value for tctPA, in the presence of stimulater Fibrin P (treated with plasmin-Sepharose)	Homo sapiens
0.000341	-	D-Ile-Pro-Arg-p-nitroanilide	value for sctPA, in the presence of stimulater Fibrin C (with cleaved C-terminal lysines)	Homo sapiens
0.000362	-	D-Ile-Pro-Arg-p-nitroanilide	value for tctPA, in the presence of stimulater Fibrin C (with cleaved C-terminal lysines)	Homo sapiens
0.694	-	D-Ile-Pro-Arg-p-nitroanilide	value for sctPA	Homo sapiens
0.694	-	D-Ile-Pro-Arg-p-nitroanilide	value for tctPA	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
using a lysine-sepharose column.Two chain tPA is generated from wild-type tPA by incubation with plasmin-sepharose	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-Ile-Pro-Arg-p-nitroanilide + H2O	-	Homo sapiens	D-Ile-Pro-Arg + p-nitroaniline	-	?
D-Val-Leu-Lys-p-nitroanilide + H2O	-	Homo sapiens	D-Val-Leu-Lys + p-nitroaniline	-	?

Synonyms

Synonyms	Comment	Organism
sctPA	-	Homo sapiens
single chain enzyme tPA	-	Homo sapiens
tctPA	-	Homo sapiens
two chain tPA	-	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.026	-	D-Ile-Pro-Arg-p-nitroanilide	value for tctPA, in the presence of stimulater Fibrin C (with cleaved C-terminal lysines)	Homo sapiens
0.027	-	D-Ile-Pro-Arg-p-nitroanilide	value for tctPA, in the presence of stimulater Fibrin normal	Homo sapiens
0.028	-	D-Ile-Pro-Arg-p-nitroanilide	value for tctPA, in the presence of stimulater Fibrin P (treated with plasmin-Sepharose)	Homo sapiens
0.0341	-	D-Ile-Pro-Arg-p-nitroanilide	value for sctPA, in the presence of stimulater Fibrin C (with cleaved C-terminal lysines)	Homo sapiens
0.086	-	D-Ile-Pro-Arg-p-nitroanilide	value for sctPA, in the presence of stimulater Fibrin P (treated with plasmin-Sepharose)	Homo sapiens
0.087	-	D-Ile-Pro-Arg-p-nitroanilide	value for sctPA, in the presence of stimulater Fibrin normal	Homo sapiens
7.3	-	D-Ile-Pro-Arg-p-nitroanilide	value for sctPA	Homo sapiens
10.7	-	D-Ile-Pro-Arg-p-nitroanilide	value for tctPA	Homo sapiens

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Release 2023.1 (January 2023)