Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cyanogen bromide-treated fibrinogen (CNBr-Fbg) | CNBr-Fbg (50 microgram/ml) give a 10-fold enhancement of activation, and addition of 2.86 microg/ml oversulfated chondroitin-6-sulfate or oversulfated fucoidan amplified this to 15-fold. A 25-fold to 35-fold enhancement of activation of glutamic plasminogen by tPA is obtained when 2.86 microgram/ml oversulfated compounds are combined with 16.2 mmol/l lysine and 50 microgram/ml CNBr-Fbg | Homo sapiens | |
L-lysine | addition of 16.2 mmol/l L-lysine give 3fold to 4fold enhancement of activation, which is further enhanced to 5fold to 6fold by addition of 2.86 microgram/ml oversulfated chondroitin-6-sulfate or oversulfated fucoidan | Homo sapiens | |
oversulfated chondroitin-6-sulfate | addition of 28.6 microgram/ml gives 2-fold to 4-fold increase in the rate of enhancement of activation of glutamic plasminogen by tPA using 0.05 mol/l Tris buffer (pH 7.35) containing NaCl (0.9%) | Homo sapiens | |
oversulfated fucoidan | addition of 28.6 microgram/ml gives 2-fold to 4-fold increase in the rate of enhancement of activation of glutamic plasminogen by tPA using 0.05 mol/l Tris buffer (pH 7.35) containing NaCl (0.9%) | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | recombinant human tissue-type plasminogen activator derivative (r-PA) (deletion mutant lacking the 4th-175th amino acid residues of t-PA), fused with thioredoxin (Trx), is expressed in Escherichia coli. Trx-r-PA fusion protein is almost completely expressed in the form of inclusion bodies and without activity. An optimized on-column refolding process for Trx-r-PA inclusion bodies is established: The collected Trx-r-PA inclusion bodies are dissolved in 6 M guanidine hydrochloride, and the denatured protein is separated from dithiothreitol and guanidine hydrochloride with a G25 column and simultaneously dissolved in 8 M urea containing oxidized glutathione. Refolding of Trx-r-PA protein on Sephacryl S-200 column with a decreasing urea gradient combined with two-stage temperature control is employed, and the activity recovery of refolded protein is increased from 3.6 to 13.8% in comparison with the usual dilution refolding | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Glu-Phe-Lys-p-nitroanilide + H2O | - |
Homo sapiens | D-Glu-Phe-Lys + p-nitroaniline | - |
? |
Synonyms | Comment | Organism |
---|---|---|
human tissue-type plasminogen activator | - |
Homo sapiens |
tPA | - |
Homo sapiens |