Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.21.68 extracted from

  • Johnsen, L.B.; Ravn, P.; Berglund, L.; Petersen, T.E.; Rasmussen, L.K.; Heegaard, C.W.; Rasmussen, J.T.; Benfeldt, C.; Fedosov, S.N.
    A refined kinetic analysis of plasminogen activation by recombinant bovine tissue-type plasminogen activator indicates two interconvertible activator forms (1998), Biochemistry, 37, 12631-12639.
    View publication on PubMed
Search for more literature for 3.4.21.68

Activating Compound

Activating Compound Comment Organism Structure
fibrinogen activation induced by Homo sapiens
fibrinogen activation induced by Bos taurus

Cloned(Commentary)

Cloned (Comment) Organism
heterologously expressed in the methylotrophic yeast Pichia pastoris GS115 Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
epsilon-aminocaproic acid
-
 Bos taurus
plasminogen activator inhibitor PAI-1
-
 Bos taurus
plasminogen activator inhibitor PAI-2
-
 Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0001
-
 plasminogen pH 7.4, 37°C Homo sapiens
0.0001
-
 plasminogen pH 7.4, 37°C Bos taurus
0.005
-
 plasminogen pH 7.4, 37°C, absence of fibrinogen Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 naked tPA amino acid chain Bos taurus
68000
-
 natively glycosylated tPA Homo sapiens
80000
-
 mass spectroscopy, SDS-PAGE Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
plasminogen + H2O Homo sapiens single-chain tPA mediated activation plasmin + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
 bovine
-
Homo sapiens
-
 human
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens
-
 Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
plasminogen + H2O single-chain tPA mediated activation Homo sapiens plasmin + ?
-
 ?
plasminogen + H2O
-
 Bos taurus ?
-
 ?
Val-Leu-Lys-p-nitroanilide + H2O i.e. S-2251 Bos taurus p-nitroaniline + ?
-
 ?

Synonyms

Synonyms Comment Organism
Tissue-type plasminogen activator
-
 Homo sapiens
Tissue-type plasminogen activator
-
 Bos taurus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0025
-
 plasminogen pH 7.4, 37°C, absence of fibrinogen Bos taurus