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Literature summary for 3.4.21.68 extracted from

  • Itagaki, Y.; Yasuda, H.; Morinaga, T.; Mitsuda, S.; Higashio, K.
    Purification and characterization of tissue plasminogen activator secreted by human embryonic lung diploid fibroblasts, IMR-90 cells [published erratum appears in Agric Biol Chem 1991 Jun;55(6):A10] (1991), Agric. Biol. Chem., 55, 1225-1232.
    View publication on PubMed
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Inhibitors

Inhibitors Comment Organism Structure
Cd2+ inhibition of amidolytic activity Homo sapiens
Co2+ inhibition of amidolytic activity Homo sapiens
Cu2+ inhibition of amidolytic activity Homo sapiens
DFP
-
 Homo sapiens
Gabexate
-
 Homo sapiens
Hg2+ inhibition of amidolytic activity Homo sapiens
leupeptin
-
 Homo sapiens
mesilate
-
 Homo sapiens
Ni2+ inhibition of amidolytic activity Homo sapiens
p-aminobenzamidine
-
 Homo sapiens
Zn2+ inhibition of amidolytic activity Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000017
-
 Peptide S-2288
-
 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
69000 72000 human, gel filtration Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
fibroblast embryonic lung diploid cells, IMR-90 cells, ATCC, CCL186 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Peptide S-2288 + H2O chromogenic substrate Homo sapiens ?
-
 ?
plasminogen + H2O
-
 Homo sapiens plasmin + ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 64000-68000, human, SDS-PAGE Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
41
-
-
 Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
95
-
 5 min, stable Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
7 10 7: about 85% of activity maximum, 10: about 80% of activity maximum Homo sapiens

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 10 stable Homo sapiens