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Literature summary for 3.4.21.64 extracted from

  • Sajnani, G.; Requena, J.R.
    Prions, proteinase K and infectivity (2012), Prion, 6, 430-432.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.21.64

Application

Application Comment Organism
diagnostics prion disease diagnosis relies on the relative resistance of sensitive prion protein Sc, PtPSc, to the non-specific protease proteinase K in brain samples to discriminate between resistant and senstive prions, PrPC and PrPSc, in combination with immunological detection of the main enzyme-resistant part of PrPSc (PrP27-30) Parengyodontium album

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
human sensitive prion protein Sc + H2O Parengyodontium album degradation, pathogenic isoform, sensitive prion protein complexes show higher molecular weight than resistant prions ?
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 ?
additional information Parengyodontium album resistant and sensitive prion protein fractions, obtained by limited proteolysis and mass spectrometry, show that both have similar enzyme-cleavage maps and therefore seems to share the same basic architecture. In vivo proteinase K-resistance of prions may not be the rule but the exception ?
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 ?

Organism

Organism UniProt Comment Textmining
Parengyodontium album P06873
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-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
human sensitive prion protein Sc + H2O degradation Parengyodontium album ?
-
 ?
human sensitive prion protein Sc + H2O degradation, pathogenic isoform, sensitive prion protein complexes show higher molecular weight than resistant prions Parengyodontium album ?
-
 ?
additional information resistant and sensitive prion protein fractions, obtained by limited proteolysis and mass spectrometry, show that both have similar enzyme-cleavage maps and therefore seems to share the same basic architecture. In vivo proteinase K-resistance of prions may not be the rule but the exception Parengyodontium album ?
-
 ?

Synonyms

Synonyms Comment Organism
Proteinase K
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 Parengyodontium album