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Literature summary for 3.4.21.64 extracted from
- Investigations on the activation of recombinant microbial pro-transglutaminase: in contrast to proteinase K, dispase removes the histidine-tag (2012), Amino Acids, 42, 997-1006.
Application
| Application | Comment | Organism |
|---|---|---|
| biotechnology | proteinase K is successfully applied for the activation of purified pro-recombinant transglutaminase either as free or immobilized enzyme and the free enzyme is also applicable directly in the crude cell extract of Escherichia coli. Proteinase K enables a simple two-step activation/purification procedure resulting in protease-free and almost pure transglutaminase preparations | Parengyodontium album |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| phenylmethylsulfonyl fluoride | - |
Parengyodontium album |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Parengyodontium album | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pro-recombinant transglutaminase + H2O | successful cleavage at the pro-sequence | Parengyodontium album | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Proteinase K | - |
Parengyodontium album |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Parengyodontium album |
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Release 2023.1 (January 2023)
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