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Literature summary for 3.4.21.64 extracted from

  • Larsen, A.N.; Moe, E.; Helland, R.; Gjellesvik, D.R.; Willassen, N.P.
    Characterization of a recombinantly expressed proteinase K-like enzyme from a psychrotrophic Serratia sp (2006), FEBS J., 273, 47-60.
    View publication on PubMed
Search for more literature for 3.4.21.64

Cloned(Commentary)

Cloned (Comment) Organism
-
 Parengyodontium album
-
 Serratia sp.

Inhibitors

Inhibitors Comment Organism Structure
EDTA 22°C, or 37°C, not inhibitory, 50°C, 50% inhibition Parengyodontium album
EDTA 22°C, not inhibitory, 37%, 60% residual activity, 50°C, complete inhibition Serratia sp.
additional information not inhibitory: sodium dodecylsulfate Parengyodontium album
additional information not inhibitory: sodium dodecylsulfate Serratia sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.46
-
 succinyl-AAPF-4-nitroanilide pH 8.0, 22°C Parengyodontium album
0.48
-
 succinyl-AAPF-4-nitroanilide pH 8.0, 12°C Parengyodontium album
0.52
-
 succinyl-AAPF-4-nitroanilide pH 8.0, 37°C Parengyodontium album
2.36
-
 succinyl-AAPF-4-nitroanilide pH 8.0, 12°C Serratia sp.
2.46
-
 succinyl-AAPF-4-nitroanilide pH 8.0, 22°C Serratia sp.
2.76
-
 succinyl-AAPF-4-nitroanilide pH 8.0, 37°C Serratia sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
 x * 65500, precursor-protein, calculated, x * 34000, mature protein, SDS-PAGE Serratia sp.
65500
-
 x * 65500, precursor-protein, calculated, x * 34000, mature protein, SDS-PAGE Serratia sp.

Organism

Organism UniProt Comment Textmining
Parengyodontium album
-
-
-
Serratia sp.
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification sequence consists of preproregion, catalytic domain and two C-terminal domains. Catalytic domain with the two C-terminal domains is an active protein of 56 kDa that converts at 50°C by autolytic cleavage to mature 34 kDa protein Serratia sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
88.6
-
-
 Parengyodontium album
88.6
-
-
 Serratia sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
succinyl-AAPF-4-nitroanilide + H2O
-
 Parengyodontium album ?
-
 ?
succinyl-AAPF-4-nitroanilide + H2O
-
 Serratia sp. ?
-
 ?

Subunits

Subunits Comment Organism
? x * 65500, precursor-protein, calculated, x * 34000, mature protein, SDS-PAGE Serratia sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
 Serratia sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 30 min, about 90% residual activity even in presence of 1% sodium dodecylsulfate Serratia sp.
50
-
 30 min, 19% residual activity in presence of 1% sodium dodecylsulfate Parengyodontium album
50
-
 30 min, 90% residual activity even in presence of 1% sodium dodecylsulfate Serratia sp.
70
-
 half-life 30 min Parengyodontium album
70
-
 half-life 19 min Serratia sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
51
-
 succinyl-AAPF-4-nitroanilide pH 8.0, 12°C Parengyodontium album
88
-
 succinyl-AAPF-4-nitroanilide pH 8.0, 22°C Parengyodontium album
175
-
 succinyl-AAPF-4-nitroanilide pH 8.0, 12°C Serratia sp.
180
-
 succinyl-AAPF-4-nitroanilide pH 8.0, 37°C Parengyodontium album
364
-
 succinyl-AAPF-4-nitroanilide pH 8.0, 22°C Serratia sp.
827
-
 succinyl-AAPF-4-nitroanilide pH 8.0, 37°C Serratia sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Parengyodontium album
10.5
-
-
 Serratia sp.

pH Range

pH Minimum pH Maximum Comment Organism
8 9.5
-
 Parengyodontium album
8 11
-
 Serratia sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 12
-
 Parengyodontium album
5.5 9.5
-
 Serratia sp.