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Literature summary for 3.4.21.61 extracted from

  • Kobayashi, H.; Takahashi, E.; Oguma, K.; Fujii, Y.; Yamanaka, H.; Negishi, T.; Arimoto-Kobayashi, S.; Tsuji, T.; Okamoto, K.
    Cleavage specificity of the serine protease of Aeromonas sobria, a member of the kexin family of subtilases (2006), FEMS Microbiol. Lett., 256, 165-170.
    View publication on PubMed
Search for more literature for 3.4.21.61

Application

Application Comment Organism
degradation ASP preferentially cleaves the peptide bond following two basic residues, one of which is Lys, but not the bond following a single basic residue. Tertiary structure around the catalytic domain of ASP resembles, but is not identical to that of furin Aeromonas sobria

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
 1 * 65000, SDS-PAGE Aeromonas sobria

Organism

Organism UniProt Comment Textmining
Aeromonas sobria
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by successive column chromatographies Aeromonas sobria

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-pyroglutamyl-Arg-Thr-Lys-Arg-4-methylcoumaryl-7-amide + H2O
-
 Aeromonas sobria L-pyroglutamyl-Arg-Thr-Lys-Arg + 7-amino-4-methyl-coumarin
-
 ?
additional information FRETS-25Xaa libraries as substrates. Best FRETS contains Lys at position Xaa (FRETS-25K). No cleavage of succinyl-Ala-Ala-Pro-Phe-4-methylylcoumaryl-7-amide, t-butyloxycarbonyl-Gly-Arg-Arg-4-methylylcoumaryl-7-amide, Z-His-Glu-Lys-4-methylylcoumaryl-7-amide, and t-butyloxycarbonyl-Phe-Ser-Arg-4-methylylcoumaryl-7-amide Aeromonas sobria ?
-
 ?
prekallikrein cleaved into four fragments by ASP, protein cleaved at specific sequences Aeromonas sobria ?
-
 ?
t-butyloxycarbonyl-Arg-Val-Arg-Arg-4-methylcoumaryl-7-amide + H2O
-
 Aeromonas sobria t-butyloxycarbonyl-Arg-Val-Arg-Arg + 7-amino-4-methyl-coumarin
-
 ?
t-butyloxycarbonyl-Glu-Lys-Lys-4-methylcoumaryl-7-amide + H2O best substrate Aeromonas sobria t-butyloxycarbonyl-Glu-Lys-Lys + 7-amino-4-methyl-coumarin
-
 ?
t-butyloxycarbonyl-Gly-Lys-Arg-4-methylcoumaryl-7-amide + H2O
-
 Aeromonas sobria t-butyloxycarbonyl-Gly-Lys-Arg + 7-amino-4-methyl-coumarin
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 65000, SDS-PAGE Aeromonas sobria

Synonyms

Synonyms Comment Organism
ASP
-
 Aeromonas sobria