Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 5 mM, complete inhibition | Bothrops cotiara | |
additional information | not inhibitory: phenylmethyl sulfonylfluoride | Bothrops cotiara | |
S2238 | noncompetitive | Bothrops cotiara |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0127 | - |
prothrombin | 22°C, pH 7.4, presence of S2238 | Bothrops cotiara | |
0.0177 | - |
prothrombin | 22°C, pH 7.4 | Bothrops cotiara |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required | Bothrops cotiara |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
22931 | - |
x * 22931, mass spectroscopy, x * 23000, SDS-PAGE | Bothrops cotiara |
23000 | - |
x * 22931, mass spectroscopy, x * 23000, SDS-PAGE | Bothrops cotiara |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bothrops cotiara | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bothrops cotiara |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
venom | - |
Bothrops cotiara | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
prothrombin + H2O | cleavage at positions Arg271-Thr272 and Arg320-Ile321 | Bothrops cotiara | alpha-thrombin + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 22931, mass spectroscopy, x * 23000, SDS-PAGE | Bothrops cotiara |
Synonyms | Comment | Organism |
---|---|---|
cotiaractivase | - |
Bothrops cotiara |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00015 | - |
prothrombin | 22°C, pH 7.4, presence of S2238 | Bothrops cotiara | |
0.00027 | - |
prothrombin | 22°C, pH 7.4 | Bothrops cotiara |