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Literature summary for 3.4.21.60 extracted from

  • Speijer, H.; Govers-Riemslag, J.W.P.; Zwaal, R.F.A.; Rosing, J.
    Prothrombin activation by an activator from the venom of Oxyuranus scutellatus (Taipan snake) (1986), J. Biol. Chem., 261, 13258-13267.
    View publication on PubMed
Search for more literature for 3.4.21.60

Activating Compound

Activating Compound Comment Organism Structure
Phospholipid + Ca2+, stimulates Oxyuranus scutellatus

Inhibitors

Inhibitors Comment Organism Structure
NaSCN bovine factor Va restores activity; reduces ability to activate prothrombin but has no effect on amidolytic activity Oxyuranus scutellatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.166
-
 prothrombin
-
 Oxyuranus scutellatus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ + phospholipid, stimulates Oxyuranus scutellatus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
 x * 110000 + x * 80000 + 2 disulfide-linked polypeptides of MW 30000 (one or both of these contain the active site), Oxyuranus scutellatus, SDS-PAGE after reduction with 2-mercaptoethanol Oxyuranus scutellatus
110000
-
 x * 110000 + x * 80000 + 2 disulfide-linked polypeptides of MW 30000 (one or both of these contain the active site), Oxyuranus scutellatus, SDS-PAGE after reduction with 2-mercaptoethanol Oxyuranus scutellatus
300000
-
 Oxyuranus scutellatus, nondenaturing PAGE Oxyuranus scutellatus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
prothrombin + H2O Oxyuranus scutellatus
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
More
-
 similar enzymes from: Pseudonaja textilis textilis, Oxyuranus microlepidotus, Demansia nuchalis affinis
-
Oxyuranus scutellatus
-
 Taipan snake
-

Source Tissue

Source Tissue Comment Organism Textmining
venom
-
 Oxyuranus scutellatus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Factor Xa-specific substrates + H2O
-
 Oxyuranus scutellatus ?
-
 ?
additional information amidolytic activity Oxyuranus scutellatus ?
-
 ?
prothrombin + H2O conversion in absence of coagulation factor Va Oxyuranus scutellatus thrombin + meizothrombin
-
 ?
prothrombin + H2O cleavage of Arg274-Thr275 and Arg323-Ile324 Oxyuranus scutellatus thrombin + meizothrombin
-
 ?
prothrombin + H2O
-
 Oxyuranus scutellatus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 110000 + x * 80000 + 2 disulfide-linked polypeptides of MW 30000 (one or both of these contain the active site), Oxyuranus scutellatus, SDS-PAGE after reduction with 2-mercaptoethanol Oxyuranus scutellatus