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Literature summary for 3.4.21.6 extracted from
- Contribution of amino acid region 659-663 of Factor Va heavy chain to the activity of factor Xa within prothrombinase (2010), Biochemistry, 49, 8520-8534.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Factor Va | interaction of factor Va with factor Xa on a membrane or cell surface in the presence of divalent metal ions and formation of the prothrombinase complex results in the reversal of the order of cleavage and a 300000fold increase in the catalytic efficiency of factor Xa for thrombin generation, structural contribution of the acidic COOH-terminal region of factor Va heavy chain to factor Xa activity within prothrombinase, overview. Functional properties and kinetics of different recombinant factor Va molecules, wild-type and mutants. Mutant factor VaDELTA659-663, factor Va5K, and factor V5A display 3.5, 11, and 24fold less clotting activity, respectively, than wild-type factor Va. Wild-type Va has a similar affinity for the factor Xa as its plasma counterpart | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cell surface | - |
Homo sapiens | 9986 | - |
| membrane | - |
Homo sapiens | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activates | Homo sapiens |  |
| additional information | divalent cations are required | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| prothrombin + H2O | Homo sapiens | activation, a first cleavage of prothrombin by prothrombinase at Arg320 produces the active intermediate meizothrombin, while the second cleavage at Arg271 produces thrombin | thrombin + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
rate of activation of native plasma-derived prothrombin in presence of prothrombinase assembled with different recombinant factor Va species, overview | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| prothrombin + H2O | - |
Homo sapiens | thrombin + ? | - |
? | |
| prothrombin + H2O | activation, a first cleavage of prothrombin by prothrombinase at Arg320 produces the active intermediate meizothrombin, while the second cleavage at Arg271 produces thrombin | Homo sapiens | thrombin + ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| factor Xa | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Homo sapiens |
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Release 2023.1 (January 2023)
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