Application | Comment | Organism |
---|---|---|
medicine | potent and selective inhibitor of tryptase will be an essential tool to determine the role of tryptase in inflammation and other processes, tryptase plays a role in asthma and other allergic and inflammatory skin diseases like psoriasis and atopic dermatitis | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
tryptases alpha and beta/II cDNA cloned and expressed in baculovirus-infected high five TM insect cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D215G | site-directed mutagenesis, derivative of tryptase alpha and beta/II | Homo sapiens |
R3Q | site-directed mutagenesis, derivative of tryptase beta/II | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diisopropylfluorophosphate | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.049 | - |
N-p-tosyl-Gly-Pro-Arg-p-nitroanilide | pH 7.4, 37°C, tryptase beta/II | Homo sapiens | |
0.12 | - |
N-p-tosyl-Gly-Pro-Arg-p-nitroanilide | pH 7.4, 37°C, D215G mutant | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
human | - |
Purification (Comment) | Organism |
---|---|
recombinant tryptases alpha and beta/II | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
fibrinogen + H2O | - |
Homo sapiens | fibrinogen fragments | - |
? | |
additional information | Ile-Phe-Lys-p-nitroanilide, N-acetyl-Ile-Glu-Ala-Arg-p-nitroanilide, N-benzoyl-Arg-p-nitroanilide, N-benzoyl-Ile-Glu-Gly-Arg-p-nitroanilide, N-benzoyl-Phe-Val-Arg-p-nitroanilide, or N-benzoyl-Pro-Phe-Arg-p-nitroanilide are no substrates | Homo sapiens | ? | - |
? | |
N-p-tosyl-Gly-Pro-Arg-p-nitroanilide + H2O | synthetic peptide substrate, mutant enzyme, expressed in insect cells | Homo sapiens | ? | - |
? | |
N-p-tosyl-Gly-Pro-Lys-p-nitroanilide + H2O | - |
Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
human tryptase alpha | - |
Homo sapiens |
human tryptase beta/2 | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.9 | - |
N-p-tosyl-Gly-Pro-Arg-p-nitroanilide | pH 7.4, 37°C, D215G mutant | Homo sapiens | |
21 | - |
N-p-tosyl-Gly-Pro-Arg-p-nitroanilide | pH 7.4, 37°C, tryptase beta/II | Homo sapiens |