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Literature summary for 3.4.21.59 extracted from

  • Huang, C.; Li, L.; Krilis, S.A.; Chanasyk, K.; Tang, Y.; Li, Z.; Hunt, J.E.; Stevens, R.L.
    Human tryptases alpha and beta/II are functionally distinct due, in part, to a single amino acid difference in one of the surface loops that forms the substrate-binding cleft (1999), J. Biol. Chem., 274, 19670-19676.
    View publication on PubMed

Application

Application Comment Organism
medicine potent and selective inhibitor of tryptase will be an essential tool to determine the role of tryptase in inflammation and other processes, tryptase plays a role in asthma and other allergic and inflammatory skin diseases like psoriasis and atopic dermatitis Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
tryptases alpha and beta/II cDNA cloned and expressed in baculovirus-infected high five TM insect cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
D215G site-directed mutagenesis, derivative of tryptase alpha and beta/II Homo sapiens
R3Q site-directed mutagenesis, derivative of tryptase beta/II Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
diisopropylfluorophosphate
-
Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.049
-
N-p-tosyl-Gly-Pro-Arg-p-nitroanilide pH 7.4, 37°C, tryptase beta/II Homo sapiens
0.12
-
N-p-tosyl-Gly-Pro-Arg-p-nitroanilide pH 7.4, 37°C, D215G mutant Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
human
-

Purification (Commentary)

Purification (Comment) Organism
recombinant tryptases alpha and beta/II Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
fibrinogen + H2O
-
Homo sapiens fibrinogen fragments
-
?
additional information Ile-Phe-Lys-p-nitroanilide, N-acetyl-Ile-Glu-Ala-Arg-p-nitroanilide, N-benzoyl-Arg-p-nitroanilide, N-benzoyl-Ile-Glu-Gly-Arg-p-nitroanilide, N-benzoyl-Phe-Val-Arg-p-nitroanilide, or N-benzoyl-Pro-Phe-Arg-p-nitroanilide are no substrates Homo sapiens ?
-
?
N-p-tosyl-Gly-Pro-Arg-p-nitroanilide + H2O synthetic peptide substrate, mutant enzyme, expressed in insect cells Homo sapiens ?
-
?
N-p-tosyl-Gly-Pro-Lys-p-nitroanilide + H2O
-
Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
human tryptase alpha
-
Homo sapiens
human tryptase beta/2
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.9
-
N-p-tosyl-Gly-Pro-Arg-p-nitroanilide pH 7.4, 37°C, D215G mutant Homo sapiens
21
-
N-p-tosyl-Gly-Pro-Arg-p-nitroanilide pH 7.4, 37°C, tryptase beta/II Homo sapiens