Inhibitors | Comment | Organism | Structure |
---|---|---|---|
p-Aminophenylmethanesulfonyl fluoride | lung enzyme is inhibited over 3times faster than the skin enzyme | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | overview: kinetic constant for the hydrolysis of synthetic thioester substrates and 4-nitroanilide substrates by the lung and by the skin enzyme | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | the preference of the skin tryptase for substrates with 2 terminal basic residues indicates that this enzyme could process prohormones and proproteins which contain this structural feature at the cleavage site | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
lung | - |
Homo sapiens | - |
skin | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Benzoyl-L-Lys-Gly-L-Arg 4-nitroanilide + H2O | best of the substrates screened | Homo sapiens | Benzoyl-L-Lys-Gly-L-Arg + 4-nitroaniline | - |
? | |
Benzyloxycarbonyl-Glu-Phe-Arg 4-nitroanilide + H2O | best substrate for lung enzyme | Homo sapiens | Benzyloxycarbonyl-Glu-Phe-Arg + 4-nitroaniline | - |
? | |
Benzyloxycarbonyl-Lys-Arg-S-CH2(CH3)2 + H2O | best substrate for skin enzyme | Homo sapiens | Benzyloxycarbonyl-Lys-Arg + S-CH2(CH3)2 | - |
? | |
additional information | skin and lung enzymes have both extended substrate-binding sites and proline residues at P3 substantially decrease kcat/Km, both enzymes prefer the tripeptide 4-nitroanilides with a P2 Gly residue over Phe, both favour the substrate benzyloxycarbonyl-Lys-Gly-Arg 4-nitroanilide over similar substrates containing six other representive amino acid residues at P3 | Homo sapiens | ? | - |
? | |
additional information | the preference of the skin tryptase for substrates with 2 terminal basic residues indicates that this enzyme could process prohormones and proproteins which contain this structural feature at the cleavage site | Homo sapiens | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | overview: kinetic constant for the hydrolysis of synthetic thioester substrates and 4-nitroanilide substrates by the lung and by the skin enzyme | Homo sapiens |