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Literature summary for 3.4.21.59 extracted from

  • Tanaka, T.; McRae, B.J.; Cho, K.; Cook, R.; Fraki, J.E.; Johnson, D.A.; Powers, J.C.
    Mammalian tissue trypsin-like enzymes. Comparative reactivities of human skin tryptase, human lung tryptase, and bovine trypsin with peptide 4-nitroanilide and thioester substrates (1983), J. Biol. Chem., 258, 13552-13557.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
p-Aminophenylmethanesulfonyl fluoride lung enzyme is inhibited over 3times faster than the skin enzyme Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information overview: kinetic constant for the hydrolysis of synthetic thioester substrates and 4-nitroanilide substrates by the lung and by the skin enzyme Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens the preference of the skin tryptase for substrates with 2 terminal basic residues indicates that this enzyme could process prohormones and proproteins which contain this structural feature at the cleavage site ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
lung
-
Homo sapiens
-
skin
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Benzoyl-L-Lys-Gly-L-Arg 4-nitroanilide + H2O best of the substrates screened Homo sapiens Benzoyl-L-Lys-Gly-L-Arg + 4-nitroaniline
-
?
Benzyloxycarbonyl-Glu-Phe-Arg 4-nitroanilide + H2O best substrate for lung enzyme Homo sapiens Benzyloxycarbonyl-Glu-Phe-Arg + 4-nitroaniline
-
?
Benzyloxycarbonyl-Lys-Arg-S-CH2(CH3)2 + H2O best substrate for skin enzyme Homo sapiens Benzyloxycarbonyl-Lys-Arg + S-CH2(CH3)2
-
?
additional information skin and lung enzymes have both extended substrate-binding sites and proline residues at P3 substantially decrease kcat/Km, both enzymes prefer the tripeptide 4-nitroanilides with a P2 Gly residue over Phe, both favour the substrate benzyloxycarbonyl-Lys-Gly-Arg 4-nitroanilide over similar substrates containing six other representive amino acid residues at P3 Homo sapiens ?
-
?
additional information the preference of the skin tryptase for substrates with 2 terminal basic residues indicates that this enzyme could process prohormones and proproteins which contain this structural feature at the cleavage site Homo sapiens ?
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information overview: kinetic constant for the hydrolysis of synthetic thioester substrates and 4-nitroanilide substrates by the lung and by the skin enzyme Homo sapiens