Inhibitors | Comment | Organism | Structure |
---|---|---|---|
alpha1-antitrypsin | - |
Rattus norvegicus | |
antipain | - |
Rattus norvegicus | |
Aprotinin | - |
Rattus norvegicus | |
diisopropyl fluorophosphate | - |
Rattus norvegicus | |
leupeptin | - |
Rattus norvegicus | |
Soybean trypsin inhibitor | - |
Rattus norvegicus | |
Trypstatin | inhibition above pH 7.5 | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mast cell granule | - |
Rattus norvegicus | 42629 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
CaCl2 | tryptase free from trypstatin is activated by 10 mM CaCl2, but tryptase associated with trypstatin is not | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
MW of the complex of tryptase and trypstatin: 144000, rat, gel filtration, MW of trypstatin is 7600 on gel filtration | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Rattus norvegicus | enzyme plays a crucial role in the process of degranulation | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
with an associated protein (trypstatin) that inhibits the protease activity above pH 7.5 (enzyme from peritoneal mast cells) | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mast cell | peritoneal | Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzyme plays a crucial role in the process of degranulation | Rattus norvegicus | ? | - |
? | |
N-tert-Butyloxycarbonyl-Ile-Glu-Gly-Arg 4-methylcoumarin 7-amide + H2O | rapid hydrolysis by free enzyme, scarcely hydrolyzed by the enzyme associated with trypstatin | Rattus norvegicus | N-tert-Butyloxycarbonyl-Ile-Glu-Gly-Arg + 7-amino-4-methylcoumarin | - |
? | |
N-tert-Butyloxycarbonyl-Phe-Ser-Arg 4-methylcoumarin 7-amide + H2O | - |
Rattus norvegicus | N-tert-Butyloxycarbonyl-Phe-Ser-Arg + 7-amino-4-methylcoumarin | - |
? | |
N-tert-Butyloxycarbonyl-Val-Pro-Arg 4-methylcoumarin 7-amide + H2O | - |
Rattus norvegicus | N-tert-Butyloxycarbonyl-Val-Pro-Arg + 7-amino-4-methylcoumarin | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
tryptase associated with trypstatin | Rattus norvegicus |
8.5 | - |
tryptase free of trypstatin | Rattus norvegicus |