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Literature summary for 3.4.21.59 extracted from

  • Kido, H.; Fukusen, N.; Katunuma, N.
    Chymotrypsin- and trypsin-type serine proteases in rat mast cells: properties and functions (1985), Arch. Biochem. Biophys., 239, 436-443.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
alpha1-antitrypsin
-
Rattus norvegicus
antipain
-
Rattus norvegicus
Aprotinin
-
Rattus norvegicus
diisopropyl fluorophosphate
-
Rattus norvegicus
leupeptin
-
Rattus norvegicus
Soybean trypsin inhibitor
-
Rattus norvegicus
Trypstatin inhibition above pH 7.5 Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
mast cell granule
-
Rattus norvegicus 42629
-

Metals/Ions

Metals/Ions Comment Organism Structure
CaCl2 tryptase free from trypstatin is activated by 10 mM CaCl2, but tryptase associated with trypstatin is not Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
MW of the complex of tryptase and trypstatin: 144000, rat, gel filtration, MW of trypstatin is 7600 on gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Rattus norvegicus enzyme plays a crucial role in the process of degranulation ?
-
?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
with an associated protein (trypstatin) that inhibits the protease activity above pH 7.5 (enzyme from peritoneal mast cells) Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
mast cell peritoneal Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme plays a crucial role in the process of degranulation Rattus norvegicus ?
-
?
N-tert-Butyloxycarbonyl-Ile-Glu-Gly-Arg 4-methylcoumarin 7-amide + H2O rapid hydrolysis by free enzyme, scarcely hydrolyzed by the enzyme associated with trypstatin Rattus norvegicus N-tert-Butyloxycarbonyl-Ile-Glu-Gly-Arg + 7-amino-4-methylcoumarin
-
?
N-tert-Butyloxycarbonyl-Phe-Ser-Arg 4-methylcoumarin 7-amide + H2O
-
Rattus norvegicus N-tert-Butyloxycarbonyl-Phe-Ser-Arg + 7-amino-4-methylcoumarin
-
?
N-tert-Butyloxycarbonyl-Val-Pro-Arg 4-methylcoumarin 7-amide + H2O
-
Rattus norvegicus N-tert-Butyloxycarbonyl-Val-Pro-Arg + 7-amino-4-methylcoumarin
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
tryptase associated with trypstatin Rattus norvegicus
8.5
-
tryptase free of trypstatin Rattus norvegicus