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Literature summary for 3.4.21.53 extracted from
- Overproduction and purification of Lon protease from Escherichia coli using a maltose-binding protein fusion system (1994), Appl. Microbiol. Biotechnol., 42, 313-318.
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
overproducing strain DH5alpha harbouring plasmid pMAL-Lon | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, in 20% glycerol, 4 months | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| casein + H2O | alpha-casein | Escherichia coli | hydrolyzed casein | - |
? |  |
| Glutaryl-Ala-Ala-Phe-methoxynaphthylamide + H2O | - |
Escherichia coli | Glutaryl-Ala-Ala-Phe + methoxynaphthylamine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| multimer | SDS-PAGE | Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | ATP-dependent protease | Escherichia coli | |
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Release 2023.1 (January 2023)
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