Literature summary for 3.4.21.50 extracted from

Kishimoto, T.; Kondo, J.; Takai-Igarashi, T.; Tanaka, H.
Accurate mass comparison coupled with two endopeptidases enables identification of protein termini (2011), Proteomics, 11, 485-489.

Search for more literature for 3.4.21.50

Application

Application	Comment	Organism
analysis	method for identification of both the amino and the carboxyl termini of proteins. The method independently uses two proteases, Lys-C and peptidyl-Lys metalloendopeptidase Lys-N, to digest proteins, followed by LC-MS/MS analysis of the two digests. Terminal peptides can be identified as the amino terminal peptide of a protein in Lys-C digest is one lysine residue mass heavier than that in Lys-N digest, the carboxyl terminal peptide in Lys-N digest is one lysine residue mass heavier than that in Lys-C digest, and all internal peptides give exactly the same molecular masses in both the Lys-C and the Lys-N digest, although amino acid sequences of Lys-C and Lys-N peptides are different. Acetylation on N-terminus and protein isoforms, which have different termini, is also determined	Achromobacter lyticus

Organism

Organism	UniProt	Comment	Textmining
Achromobacter lyticus	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
commercial preparation	-	Achromobacter lyticus	-

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Release 2023.1 (January 2023)