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Literature summary for 3.4.21.50 extracted from

  • Chohnan, S.; Shiraki, K.; Yokota, K.; Ohshima, M.; Kuroiwa, N.; Ahmed, K.; Masaki, T.; Sakiyama, F.
    A second lysine-specific serine protease from Lysobacter sp. strain IB-9374 (2004), J. Bacteriol., 186, 5093-5100.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.21.50

Cloned(Commentary)

Cloned (Comment) Organism
-
 Lysobacter sp.

Inhibitors

Inhibitors Comment Organism Structure
diisopropyl fluorophosphate
-
 Lysobacter sp.
Guanidine-HCl 0.1 M, 20 min, 72% loss of activity Lysobacter sp.
N-tosyl-L-Lys chloromethylketone
-
 Lysobacter sp.
sodium dodecylsulfate 0.1%, 20 min, 70% loss of activity Lysobacter sp.
Urea 4 M, 20 min, 80% loss of activity Lysobacter sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
 x * 32000, SDS-PAGE Lysobacter sp.

Organism

Organism UniProt Comment Textmining
Lysobacter sp. Q8GQZ0 isoform LepB
-
Lysobacter sp. IB-9374 Q8GQZ0 isoform LepB
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification sequence contains a prepetide of 20 amino acids, a propeptide of 184 amino acids and a mature enzyme of 274 amino acids with a C-terminal extension peptide of 200 amino acids Lysobacter sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.3
-
 pH 9.2, 30°C Lysobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzoyl-L-Lys-4-nitroanilide + H2O
-
 Lysobacter sp. benzoyl-L-Lys + 4-nitroaniline
-
 ?
benzoyl-L-Lys-4-nitroanilide + H2O
-
 Lysobacter sp. IB-9374 benzoyl-L-Lys + 4-nitroaniline
-
 ?
Glucagon + H2O specific hydrolysis of lysyl peptide bond Lysobacter sp. ?
-
 ?
Glucagon + H2O specific hydrolysis of lysyl peptide bond Lysobacter sp. IB-9374 ?
-
 ?
L-Lys-4-nitroanilide + H2O slow reaction Lysobacter sp. L-Lys + 4-nitroaniline
-
 ?
L-Lys-4-nitroanilide + H2O slow reaction Lysobacter sp. IB-9374 L-Lys + 4-nitroaniline
-
 ?

Subunits

Subunits Comment Organism
? x * 32000, SDS-PAGE Lysobacter sp.
More isozyme of LepA, can fold into a three-dimensional structure quite similar to LepA Lysobacter sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Lysobacter sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 moderately stable up to Lysobacter sp.

pH Range

pH Minimum pH Maximum Comment Organism
7 10 more than 50% of maximum activity in this range Lysobacter sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 11 4°C, stable for at least 24 h Lysobacter sp.