Protein Variants | Comment | Organism |
---|---|---|
H210/W169F | the ratio of turnover number to Km-value for tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin as substrate is 8.6% of the wild-type ratio | Achromobacter lyticus |
H210A | the ratio of turnover number to Km-value for tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin as substrate is 80% of the wild-type ratio | Achromobacter lyticus |
H210A/W169A | the ratio of turnover number to Km-value for tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin as substrate is 0.25% of the wild-type ratio | Achromobacter lyticus |
H210K | the ratio of turnover number to Km-value for tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin as substrate is 0.02% of the wild-type ratio | Achromobacter lyticus |
H210S | the ratio of turnover number to Km-value for tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin as substrate is 1.7fold higher than the wild-type ratio | Achromobacter lyticus |
W169A | the ratio of turnover number to Km-value for tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin as substrate 0.5% of the wild-type ratio | Achromobacter lyticus |
W169F | the ratio of turnover number to Km-value for tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin as substrate is 43% of the wild-type ratio | Achromobacter lyticus |
W169H | the ratio of turnover number to Km-value for tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin as substrate is 9% of the wild-type ratio | Achromobacter lyticus |
W169L | the ratio of turnover number to Km-value for tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin as substrate is 25% of the wild-type ratio | Achromobacter lyticus |
W169V | the ratio of turnover number to Km-value for tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin as substrate is 6% of the wild-type ratio | Achromobacter lyticus |
W169Y | the ratio of turnover number to Km-value for tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin as substrate is 43% of the wild-type ratio | Achromobacter lyticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Achromobacter lyticus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin + H2O | - |
Achromobacter lyticus | tert-butyloxycarbonyl-Val-Leu-Lys + 7-amino-4-methylcoumarin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Achromobacter protease I | - |
Achromobacter lyticus |
API | - |
Achromobacter lyticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 9 | mutant enzyme H210S, hydrolysis of tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin | Achromobacter lyticus |
9 | 10 | mutant enzyme W169V, hydrolysis of tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin | Achromobacter lyticus |
9.5 | - |
wild-type enzyme, hydrolysis of tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin | Achromobacter lyticus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8 | 10 | pH 8.0: about 40% of maximal activity, pH 10.0: about 95% of maximal activity, wild-type enzyme, hydrolysis of tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin | Achromobacter lyticus |