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Literature summary for 3.4.21.50 extracted from

  • Masaki, T.; Nakamura, T.; Isono, M.; Soejima, M.
    A new proteolytic enzyme from Achromobacter lyticus M497-1 (1978), Agric. Biol. Chem., 42, 1443-1445.
No PubMed abstract available
Search for more literature for 3.4.21.50

Inhibitors

Inhibitors Comment Organism Structure
diisopropylfluorophosphate
-
 Achromobacter lyticus
Nalpha-p-tosyl-L-Lys-chloromethylketone
-
 Achromobacter lyticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
 N-benzoyl-L-Lys p-nitroanilide protease I Achromobacter lyticus
0.08
-
 Lys-p-nitroanilide protease I Achromobacter lyticus
0.32
-
 benzoyl-Lys-NH2
-
 Achromobacter lyticus
20
-
 benzoyl-Arg ethyl ester
-
 Achromobacter lyticus
35.7
-
 N-tosyl-L-Arg methyl ester protease I Achromobacter lyticus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
-
 Achromobacter lyticus

Organism

Organism UniProt Comment Textmining
Achromobacter lyticus
-
 M497-1
-
Achromobacter lyticus M497-1
-
 M497-1
-

Purification (Commentary)

Purification (Comment) Organism
-
 Achromobacter lyticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzoyl-Arg ethyl ester + H2O
-
 Achromobacter lyticus benzoyl-Arg + ethanol
-
 ?
benzoyl-Lys-NH2 + H2O
-
 Achromobacter lyticus benzoyl-Lys + NH3
-
 ?
Lys-p-nitroanilide + H2O
-
 Achromobacter lyticus Lys + p-nitroaniline
-
 ?
Lys-p-nitroanilide + H2O
-
 Achromobacter lyticus M497-1 Lys + p-nitroaniline
-
 ?
additional information the enzyme cleaves only the Lys-X bond Achromobacter lyticus ?
-
 ?
additional information the enzyme cleaves only the Lys-X bond Achromobacter lyticus M497-1 ?
-
 ?
N-benzoyl-L-Lys-p-nitroanilide + H2O
-
 Achromobacter lyticus N-benzoyl-L-Lys + p-nitroaniline
-
 ?
N-benzoyl-L-Lys-p-nitroanilide + H2O
-
 Achromobacter lyticus M497-1 N-benzoyl-L-Lys + p-nitroaniline
-
 ?
N-tosyl-L-Arg methyl ester + H2O
-
 Achromobacter lyticus N-tosyl-L-Arg + methanol
-
 ?
N-tosyl-L-Arg methyl ester + H2O
-
 Achromobacter lyticus M497-1 N-tosyl-L-Arg + methanol
-
 ?
N-tosyl-L-Lys methyl ester + H2O
-
 Achromobacter lyticus N-tosyl-L-Lys + methanol
-
 ?
N-tosyl-L-Lys methyl ester + H2O
-
 Achromobacter lyticus M497-1 N-tosyl-L-Lys + methanol
-
 ?