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Literature summary for 3.4.21.50 extracted from

  • Masaki, T.; Fujihashi, T.; Nakamura, K.; Soejima, M.
    Studies on a new proteolytic enzyme from Achromobacter lyticus M497-1. II. specificity and inhibition studies of Achromobacter protease I (1981), Biochim. Biophys. Acta, 660, 51-55.
    View publication on PubMed
Search for more literature for 3.4.21.50

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.091
-
 benzoyl-Lys methyl ester
-
 Achromobacter lyticus
0.1
-
 N-tosyl-L-Lys methyl ester protease I Achromobacter lyticus
1
-
 N-benzoyl-L-Orn methyl ester protease I Achromobacter lyticus
20
-
 benzoyl-Arg ethyl ester
-
 Achromobacter lyticus
35.7
-
 N-tosyl-L-Arg methyl ester protease I Achromobacter lyticus

Organism

Organism UniProt Comment Textmining
Achromobacter lyticus
-
 M497-1
-
Achromobacter lyticus M497-1
-
 M497-1
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ACTH + H2O hydrolysis of the bonds: Lys11-Pro12, Lys16-Lys16, Lys16-Arg17, Lys21-Val22 Achromobacter lyticus ?
-
 ?
B-chain of insulin + H2O only cleavage of the bond Lys29-Ala30 Achromobacter lyticus ?
-
 ?
B-chain of insulin + H2O only cleavage of the bond Lys29-Ala30 Achromobacter lyticus M497-1 ?
-
 ?
benzoyl-Arg ethyl ester + H2O
-
 Achromobacter lyticus benzoyl-Arg + ethanol
-
 ?
benzoyl-Lys methyl ester + H2O
-
 Achromobacter lyticus benzoyl-Lys + methanol
-
 ?
lysine vasopressin + H2O only the Lys8-Gly9 bond is hydrolyzed Achromobacter lyticus ?
-
 ?
lysine vasopressin + H2O only the Lys8-Gly9 bond is hydrolyzed Achromobacter lyticus M497-1 ?
-
 ?
additional information the enzyme cleaves only the Lys-X bond Achromobacter lyticus ?
-
 ?
additional information the enzyme cleaves only the Lys-X bond Achromobacter lyticus M497-1 ?
-
 ?
N-benzoyl-L-Orn methyl ester + H2O
-
 Achromobacter lyticus N-benzoyl-L-Orn + methanol
-
 ?
N-tosyl-L-Arg methyl ester + H2O
-
 Achromobacter lyticus N-tosyl-L-Arg + methanol
-
 ?
N-tosyl-L-Arg methyl ester + H2O
-
 Achromobacter lyticus M497-1 N-tosyl-L-Arg + methanol
-
 ?
N-tosyl-L-Lys methyl ester + H2O
-
 Achromobacter lyticus N-tosyl-L-Lys + methanol
-
 ?
Substance P + H2O hydrolysis of the bond Lys3-Pro4 Achromobacter lyticus ?
-
 ?
Substance P + H2O hydrolysis of the bond Lys3-Pro4 Achromobacter lyticus M497-1 ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.04
-
 N-tosyl-L-Arg methyl ester
-
 Achromobacter lyticus
7.41
-
 benzoyl-Arg ethyl ester
-
 Achromobacter lyticus
10.4
-
 N-benzoyl-L-Orn methyl ester
-
 Achromobacter lyticus
225
-
 benzoyl-Lys methyl ester
-
 Achromobacter lyticus
570
-
 N-tosyl-L-Lys methyl ester
-
 Achromobacter lyticus