General Stability | Organism |
---|---|
7 M urea, pH 8.0, 30°C, 20 min, 30% loss of activity | Achromobacter lyticus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | - |
Achromobacter lyticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
N-benzoyl-L-Lys p-nitroanilide | protease I | Achromobacter lyticus | |
0.08 | - |
Lys-p-nitroanilide | protease I | Achromobacter lyticus | |
0.083 | - |
N-tosyl-L-Lys methyl ester | protease Ia | Achromobacter lyticus | |
0.09 | - |
N-benzoyl-L-Lys p-nitroanilide | protease Ia | Achromobacter lyticus | |
0.1 | - |
N-tosyl-L-Lys methyl ester | protease I | Achromobacter lyticus | |
0.11 | - |
Lys-p-nitroanilide | protease Ia | Achromobacter lyticus | |
0.63 | - |
N-benzoyl-L-Orn methyl ester | protease Ia | Achromobacter lyticus | |
1 | - |
N-benzoyl-L-Orn methyl ester | protease I | Achromobacter lyticus | |
2.5 | - |
N-tosyl-L-Arg methyl ester | protease Ia | Achromobacter lyticus | |
35.7 | - |
N-tosyl-L-Arg methyl ester | protease I | Achromobacter lyticus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27000 | - |
gel filtration | Achromobacter lyticus |
30000 | - |
gel filtration | Achromobacter lyticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Achromobacter lyticus | - |
M497-1 | - |
Achromobacter lyticus | - |
protease I and protease Ia | - |
Achromobacter lyticus M497-1 | - |
M497-1 | - |
Purification (Comment) | Organism |
---|---|
protease Ia | Achromobacter lyticus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture filtrate | - |
Achromobacter lyticus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Achromobacter lyticus |
Storage Stability | Organism |
---|---|
4°C, pH 5.0-11.0, 24 h, stable | Achromobacter lyticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
B-chain of insulin + H2O | protease I1 cleaves Lys29-Ala30 bond | Achromobacter lyticus | ? | - |
? | |
B-chain of insulin + H2O | protease I1 cleaves Lys29-Ala30 bond | Achromobacter lyticus M497-1 | ? | - |
? | |
Lys-p-nitroanilide + H2O | - |
Achromobacter lyticus | Lys + p-nitroaniline | - |
? | |
Lys-p-nitroanilide + H2O | - |
Achromobacter lyticus M497-1 | Lys + p-nitroaniline | - |
? | |
additional information | no hydrolysis of: N-benzoyl-L-Arg p-nitroanilide and Arg-p-nitroanilide | Achromobacter lyticus | ? | - |
? | |
additional information | no hydrolysis of: N-benzoyl-L-Arg p-nitroanilide and Arg-p-nitroanilide | Achromobacter lyticus M497-1 | ? | - |
? | |
N-benzoyl-L-Lys-p-nitroanilide + H2O | - |
Achromobacter lyticus | N-benzoyl-L-Lys + p-nitroaniline | - |
? | |
N-benzoyl-L-Orn methyl ester + H2O | - |
Achromobacter lyticus | N-benzoyl-L-Orn + methanol | - |
? | |
N-tosyl-L-Arg methyl ester + H2O | - |
Achromobacter lyticus | N-tosyl-L-Arg + methanol | - |
? | |
N-tosyl-L-Arg methyl ester + H2O | - |
Achromobacter lyticus M497-1 | N-tosyl-L-Arg + methanol | - |
? | |
N-tosyl-L-Lys methyl ester + H2O | - |
Achromobacter lyticus | N-tosyl-L-Lys + methanol | - |
? | |
N-tosyl-L-Lys methyl ester + H2O | - |
Achromobacter lyticus M497-1 | N-tosyl-L-Lys + methanol | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
20 min, stable up to | Achromobacter lyticus |
60 | - |
20 min, complete loss of activity | Achromobacter lyticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | 8.2 | esterolytic activity | Achromobacter lyticus |
8.8 | - |
amidolytic activity | Achromobacter lyticus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 11 | 4°C, 24 h, stable | Achromobacter lyticus |