Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.21.5 extracted from

  • Gandhi, P.S.; Page, M.J.; Chen, Z.; Bush-Pelc, L.; Di Cera, E.
    Mechanism of the anticoagulant activity of thrombin mutant W215A/E217A (2009), J. Biol. Chem., 284, 24098-24105.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.21.5

Cloned(Commentary)

Cloned (Comment) Organism
-
 Mus musculus
-
 Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 100 mM MES, 30% (w/v) polyethylene glycol 400, pH 6.5 Homo sapiens
hanging drop vapor diffusion method, using either 0.2 M NH4Cl, 20% (w/v) polyethylene glycol 3350, pH 6.3, or 0.2 M NH4Cl, 14% (w/v) polyethylene glycol 3350, pH 4.6, or 0.2 M Na2SO4, 20% (w/v) polyethylene glycol 3350, pH 6.6 Mus musculus

Protein Variants

Protein Variants Comment Organism
W215A/E217A the thrombin mutant is a potent anticoagulant both in vitro and in vivo Mus musculus
W215A/E217A the thrombin mutant is a potent anticoagulant both in vitro and in vivo Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
fibrinogen + H2O Mus musculus
-
 fibrin + fibrinopeptide A + fibrinopeptide B
-
 ?
fibrinogen + H2O Homo sapiens
-
 fibrin + fibrinopeptide A + fibrinopeptide B
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Mus musculus
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
fibrinogen + H2O
-
 Mus musculus fibrin + fibrinopeptide A + fibrinopeptide B
-
 ?
fibrinogen + H2O
-
 Homo sapiens fibrin + fibrinopeptide A + fibrinopeptide B
-
 ?