Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Na+ | binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P00734 | commercial preparation | - |
Renatured (Comment) | Organism |
---|---|
denaturation by urea, 60% reversible at pH 8.0, completely reversible at pH 6.5. Na+-bound form of enzyme is more stable to urea denaturation than Na+-free form by 2 kcal/mol | Homo sapiens |