Literature summary for 3.4.21.48 extracted from

Perez, J.; Gomez, A.; Roncero, C.
Upregulation of the PRB1 gene in the Saccharomyces cerevisiae rim101Delta mutant produces proteolytic artefacts that differentially affect some proteins (2010), Yeast, 27, 575-581.

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Application

Application	Comment	Organism
synthesis	much stricter experimental protocols than those routinely used are necessary to prevent the artefactual interpretation of protein levels in strains or conditions that increase proteinase B activity. Stability of some proteins, such as Slt2p or Chs4p, but not others, is severely compromised in the rim101 mutant due to the upregulation of the PRB1 gene. Proteolytic degradation during protein processing can be almost completely prevented by an overdose of subtilisin-like protease inhibitors, such as PMSF, or by avoiding cell freezing	Saccharomyces cerevisiae

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	-	-	-

Synonyms

Synonyms	Comment	Organism
PRB1	-	Saccharomyces cerevisiae

General Information

General Information	Comment	Organism
physiological function	deletion of the rim101 gene involved in sporulation control results in upregulation of the PRB1 gene leading to higher levels of proteinase B activity. Increase in proteinase B activity results in severely compromised stability of some proteins such as Slt2p or Chs4p	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)