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Literature summary for 3.4.21.47 extracted from

  • Harris, C.L.; Pettigrew, D.M.; Lea, S.M.; Morgan, B.P.
    Decay-accelerating factor must bind both components of the complement alternative pathway C3 convertase to mediate efficient decay (2007), J. Immunol., 178, 352-359.
    View publication on PubMed
Search for more literature for 3.4.21.47

Activating Compound

Activating Compound Comment Organism Structure
properdin positive regulator of the alternative pathway, no direct interaction with the decay-accelerating factor DAF determined Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
decay-accelerating factor DAF, CD55, major site of interaction with the larger cleavage subunit complement component factor B (Bb), interaction pathway dissected, second short consensus repeat (SCR) domain of DAF (SCR2) interacts only with fragment Bb, whereas SCR4 interacts with complement component C3b, SCR3 does not directly interact with either subunit Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
complement component C3 + H2O Homo sapiens
-
 complement component C3a + complement component C3b
-
 ?
complement component C5 + H2O Homo sapiens
-
 complement component C5a + complement component C5b
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P00751
-
-

Source Tissue

Source Tissue Comment Organism Textmining
serum
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 hemolysis assay to assess inhibition by DAF mutants described, equilibrium analysis of recombinant soluble DAF mutants binding to complement component C3b shown, kinetic constants for binding of DAF mutants to the complement component C3b and fragment Bb and ability to decay AP convertase determined, interaction of DAF with properdin and decay of properdin-stabilized convertase indicated, binding kinetics of fragment Bb to recombinant soluble DAF mutants DAF1234, DAF23 and DAF34 determined, decay of AP C3 convertase by DAF and DAF mutants monitored Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
complement component C3 + H2O
-
 Homo sapiens complement component C3a + complement component C3b
-
 ?
complement component C3 + H2O interaction studies with decay-accelerating factor DAF mediating decay of the alternative pathway C3 convertase, C3bBb, but not of the inactive proenzyme C3bB Homo sapiens complement component C3a + complement component C3b
-
 ?
complement component C5 + H2O
-
 Homo sapiens complement component C5a + complement component C5b
-
 ?

Synonyms

Synonyms Comment Organism
C3 convertase
-
 Homo sapiens