Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta (DE3) cells | Megalobrama amblycephala |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
complement factor B + H2O | Megalobrama amblycephala | - |
fragment Ba + fragment Bb | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Megalobrama amblycephala | - |
blunt snout bream | - |
Purification (Comment) | Organism |
---|---|
Ni-agarose column chromatography | Megalobrama amblycephala |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
blood | low expression | Megalobrama amblycephala | - |
brain | moderate expression | Megalobrama amblycephala | - |
gill | moderate expression | Megalobrama amblycephala | - |
head kidney | - |
Megalobrama amblycephala | - |
heart | - |
Megalobrama amblycephala | - |
intestine | low expression | Megalobrama amblycephala | - |
kidney | highest expression in the kidney in healthy adults | Megalobrama amblycephala | - |
liver | - |
Megalobrama amblycephala | - |
muscle | moderate expression | Megalobrama amblycephala | - |
spleen | moderate expression | Megalobrama amblycephala | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
complement factor B + H2O | - |
Megalobrama amblycephala | fragment Ba + fragment Bb | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 27200, calculated from amino acid sequence | Megalobrama amblycephala |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Megalobrama amblycephala | calculated from amino acid sequence | - |
6.7 |
Organism | Comment | Expression |
---|---|---|
Megalobrama amblycephala | after challenged with Aeromonas hydrophila, the enzyme, at both mRNA and protein levels, is upregulated in the kidney, spleen, liver and head-kidney | up |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme shows antimicrobial activity against both gram-positive bacteria like Staphylococcus aureus and gram-negative bacteria like Aeromonas hydrophila | Megalobrama amblycephala |