Cloned (Comment) | Organism |
---|---|
expressed in HEK293-E cells | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
S183A mutant protein in complex with pro-convertase C3bB | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
H133A | catalytically inactive | Homo sapiens |
R115C | activity like wild type | Homo sapiens |
R157A | catalytically inactive | Homo sapiens |
R202A | putative interaction with Glu230 in factor B, cleavage of factor B reduced to 20%, activity against peptides threefold increased | Homo sapiens |
S183A | catalytically inactive | Homo sapiens |
V203D | very low activity | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
cation-exchange chromatography,gelfiltration | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Ac-Lys-Arg-4-nitroanilide + H2O | - |
Homo sapiens | Ac-Lys-Arg + 4-nitroaniline | - |
? | |
pro-convertase C3bB + H2O | - |
Homo sapiens | active C3 convertase C3bBb + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
factor D | complement | Homo sapiens |