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Literature summary for 3.4.21.46 extracted from

  • Toivanen, P.I.; Nieminen, T.; Viitanen, L.; Alitalo, A.; Roschier, M.; Jauhiainen, S.; Markkanen, J.E.; Laitinen, O.H.; Airenne, T.T.; Salminen, T.A.; Johnson, M.S.; Airenne, K.J.; Ylae-Herttuala, S.
    Novel vascular endothelial growth factor D (VEGF-D) variants with increased biological activity (2009), J. Biol. Chem., 284, 16037-1648.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.21.46

Cloned(Commentary)

Cloned (Comment) Organism
mutants transiently transfected into 293T cells. The native VEGF-DDELTANDELTAC and mutants expressed in a larger scale in insect cells using BVboostFG baculovirus expression system Homo sapiens

Protein Variants

Protein Variants Comment Organism
C25A has increased activity compared to the native VEGF-DDELTANDELTAC. Efficiently binds to the soluble receptor VEGFR-2/IgGFc. The C25A mutant behaves mainly as a monomeric protein on SDS-PAGE under reducing conditions but as a dimeric protein under non-reducing conditions. It induces VEGFR-2 Tyr-1175 phosphorylation Homo sapiens
C25A/P43S has biological activity comparable to that of the native protein in Ba/F3-VEGFR-2 cells Homo sapiens
C25I is mainly in a presumably covalently bound dimeric form Homo sapiens
C25L has increased activity compared to the native VEGF-DDELTANDELTAC, the C25L mutant is the most active mutant and is mainly in a presumably covalently bound dimeric form, has highest affinity to bind soluble receptor VEGFR-2/IgGFc. It induces VEGFR-2 Tyr-1175 phosphorylation Homo sapiens
C25V is mainly in a presumably covalently bound dimeric form Homo sapiens
C44A does not bind to the soluble receptor VEGFR-2/IgGFc Homo sapiens
C53A does not bind the soluble receptors VEGFR-2/IgGFc and VEGFR-3/IgGFc Homo sapiens
C53A does not bind to the soluble receptor VEGFR-2/IgGFc Homo sapiens
G51C can not induce cell survival, has increased dimer to monomer ratio Homo sapiens
P43S can not induce cell survival Homo sapiens
R22I can not induce cell survival Homo sapiens
R22I/C25L has biological activity comparable to that of the native protein in Ba/F3-VEGFR-2 cells, has increased dimer to monomer ratio Homo sapiens
R22L can not induce cell survival Homo sapiens
R22L/C25L has biological activity comparable to that of the native protein in Ba/F3-VEGFR-2 cells, has increased dimer to monomer ratio Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens O43915
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-

Purification (Commentary)

Purification (Comment) Organism
VEGF-DDELTANDELTAC and the three cysteine mutants purified by metal affinity chromatography Homo sapiens

Subunits

Subunits Comment Organism
homodimer SDS-PAGE and immunoblotting. VEGF-DDeltaNDeltaC homodimer is antiparallel and has an elongated shape but is flat at the central part. It has three subunit interface cysteines C25, C44 and C53 Homo sapiens

Synonyms

Synonyms Comment Organism
vascular endothelial growth factor
-
 Homo sapiens
VEGF-D
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 Homo sapiens