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Literature summary for 3.4.21.45 extracted from

  • Xiang, J.; Li, X.; Chen, Y.; Lu, Y.; Yu, M.; Chen, X.; Zhang, W.; Zeng, Y.; Sun, L.; Chen, S.; Sha, Z.
    Complement factor I from flatfish half-smooth tongue (Cynoglossus semilaevis) exhibited anti-microbial activities (2015), Dev. Comp. Immunol., 53, 199-209 .
    View publication on PubMed
Search for more literature for 3.4.21.45

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Cynoglossus semilaevis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
complement component C3b + H2O Cynoglossus semilaevis
-
 ?
-
 ?
complement component C4b + H2O Cynoglossus semilaevis
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Cynoglossus semilaevis A0A0H4CX09
-
-

Purification (Commentary)

Purification (Comment) Organism
His Trap column chromatography Cynoglossus semilaevis

Renatured (Commentary)

Renatured (Comment) Organism
dialysis with from 6 to 0 M urea Cynoglossus semilaevis

Source Tissue

Source Tissue Comment Organism Textmining
blood
-
 Cynoglossus semilaevis
-
brain lowest expression Cynoglossus semilaevis
-
gill
-
 Cynoglossus semilaevis
-
head kidney
-
 Cynoglossus semilaevis
-
heart
-
 Cynoglossus semilaevis
-
intestine high expression Cynoglossus semilaevis
-
liver predominant expression Cynoglossus semilaevis
-
muscle
-
 Cynoglossus semilaevis
-
ovary high expression Cynoglossus semilaevis
-
skin
-
 Cynoglossus semilaevis
-
spleen
-
 Cynoglossus semilaevis
-
stomach lowest expression Cynoglossus semilaevis
-
trunk kidney lowest expression Cynoglossus semilaevis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
complement component C3b + H2O
-
 Cynoglossus semilaevis ?
-
 ?
complement component C4b + H2O
-
 Cynoglossus semilaevis ?
-
 ?

Subunits

Subunits Comment Organism
? x * 69230, mature protein, calculated from amino acid sequence Cynoglossus semilaevis
? x * 71000, His6-tagged enzyme, SDS-PAGE Cynoglossus semilaevis
? x * 72280, calculated from amino acid sequence Cynoglossus semilaevis

Synonyms

Synonyms Comment Organism
CFI
-
 Cynoglossus semilaevis

pI Value

Organism Comment pI Value Maximum pI Value
Cynoglossus semilaevis calculated from amino acid sequence
-
 7.1

Expression

Organism Comment Expression
Cynoglossus semilaevis enzyme expression is increased after challenge with Vibrio anguillarum up

General Information

General Information Comment Organism
physiological function the enzyme plays an important role in Cynoglossus semilaevis immunity and shows broad-spectrum antimicrobial activities against the Gram-positive bacteria Staphylococcus aureus and the Gram-negative bacteria Escherichia coli, Pseudomonas aeruginosa and Shewanella putrefaciens Cynoglossus semilaevis