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Literature summary for 3.4.21.43 extracted from

  • Nagasawa, S.; Kobayashi, C.; Maki-Suzuki, T.; Yamashita, N.; Koyama, J.
    Purification and characterization of the C3 convertase of the classical pathway of human complement system by size exclusion high-performance liquid chromatography (1985), J. Biochem., 97, 493-499.
    View publication on PubMed
Search for more literature for 3.4.21.43

General Stability

General Stability Organism
complement component C2b accelerates dissociation Homo sapiens
complex C4bC2a extremely unstable Homo sapiens
dilute iodine solution stabilizes Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Iodine stabilization Homo sapiens
Mg2+ formation of the bimolecular complex C4b2a, i.e. C3 convertase Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
280000
-
 gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
complement component C3 + H2O Homo sapiens classical pathway of the complement system ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 2 distinct C3 convertases generated by 2 seperate enzyme cascade systems
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
Selective cleavage of Arg-/-Ser bond in complement component C3 alpha-chain to form C3a and C3b, and Arg-/- bond in complement component C5 alpha-chain to form C5a and C5b hydrolyses peptide bond 77 (Arg-Ser) in complement component C3, and complement component C5. Cleavage of C5 requires complement fragment C3b which binds C5 and renders it susceptible to cleavage by the C4b,C2a complex Homo sapiens
a

Source Tissue

Source Tissue Comment Organism Textmining
plasma
-
 Homo sapiens
-

Storage Stability

Storage Stability Organism
0°C, 8 d, 40% loss of activity Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
complement component C3 + H2O hydrolyzes peptide bond Arg77-Ser of the alpha-chain of C3 Homo sapiens complement component C3a + complement component C3b
-
 ?
complement component C3 + H2O classical pathway of the complement system Homo sapiens ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
24
-
 half-life: 400 min Homo sapiens
30
-
 half-life: 180 min Homo sapiens
37
-
 half-life: 60 min Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Homo sapiens

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5 9 at pH 6.5 more stable than at pH 9 Homo sapiens