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Literature summary for 3.4.21.43 extracted from

  • Krishnan, V.; Xu, Y.; Macon, K.; Volanakis, J.E.; Narayana, S.V.
    The structure of C2b, a fragment of complement component C2 produced during C3 convertase formation (2009), Acta Crystallogr. Sect. D, 65, 266-274.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
complement C2 provides catalytic activity for the C3 convertase C4bC2a Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
full-length C2 cDNA cloned into the baculovirus expression vector pACgp67A and cotransfected with BD Baculogold Bright linearized baculovirus DNA into sf9 insect cells. High-titer stocks produced in sf9 insect cells and used to infect High Five insect cells for protein expression Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
N-terminal segment C2b, by the hanging-drop vapor-diffusion method, to 1.8 A resolution. Space group P31 with unit-cell parameters a=b= 60.09 A and c = 61.69 A. Conformational changes of C2, C2a and C2b during C3 convertase C4bC2a formation Homo sapiens

Protein Variants

Protein Variants Comment Organism
C241A C2 mutation, behaves similarly to the wild-type enzyme with slightly elevated hemolytic activity Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
complement C2, by gel filtration Homo sapiens

Synonyms

Synonyms Comment Organism
C3 convertase
-
Homo sapiens
C4bC2a
-
Homo sapiens