Activating Compound | Comment | Organism | Structure |
---|---|---|---|
complement C2 | provides catalytic activity for the C3 convertase C4bC2a | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
full-length C2 cDNA cloned into the baculovirus expression vector pACgp67A and cotransfected with BD Baculogold Bright linearized baculovirus DNA into sf9 insect cells. High-titer stocks produced in sf9 insect cells and used to infect High Five insect cells for protein expression | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
N-terminal segment C2b, by the hanging-drop vapor-diffusion method, to 1.8 A resolution. Space group P31 with unit-cell parameters a=b= 60.09 A and c = 61.69 A. Conformational changes of C2, C2a and C2b during C3 convertase C4bC2a formation | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C241A | C2 mutation, behaves similarly to the wild-type enzyme with slightly elevated hemolytic activity | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
complement C2, by gel filtration | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
C3 convertase | - |
Homo sapiens |
C4bC2a | - |
Homo sapiens |