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Literature summary for 3.4.21.42 extracted from

  • Sim, R.B.
    The human complement system serine proteases C1bar and C1sbar and their proenzymes (1981), Methods Enzymol., 80, 26-42.
    View publication on PubMed

General Stability

General Stability Organism
stable at concentrations of 1-2 mg/ml Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
amidines
-
Homo sapiens
C1bar inhibitor
-
Homo sapiens
DFP
-
Homo sapiens
Guanidines
-
Homo sapiens
p-nitrophenyl-p'-guanidinobenzoate
-
Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.056 0.07 N-benzyloxycarbonyl-Tyr p-nitrophenyl ester
-
Homo sapiens
0.8
-
N-benzyloxycarbonyl-Lys p-nitrophenyl ester
-
Homo sapiens
1.7 2.8 tosyl-Arg methyl ester
-
Homo sapiens
2.5 5 benzoyl-Arg ethyl ester
-
Homo sapiens
19
-
acetyl-L-Tyr ethyl ester
-
Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
x * 56000, A chain, + x * 27000, B chain, gel filtration under denaturing conditions Homo sapiens
56000
-
x * 56000, A chain, + x * 27000, B chain, gel filtration under denaturing conditions Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens binding of C1 to certain viral activators occurs through both C1s and C1q ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification C1rbar cleaves a single Arg-Ile or Lys-Ile bond in proenzyme C1s Homo sapiens
proteolytic modification C1s is the proenzyme of C1sbar Homo sapiens
side-chain modification C1sbar A chain contains 6.5% carbohydrate per weight, C1sbar B chain contains 7.5% carbohydrate per weight Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
C1s and C1sbar Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Benzoyl-Arg ethyl ester + H2O
-
Homo sapiens ?
-
?
complement component C2 + H2O cleavage of a single bond, likely to be Lys-Lys or Arg-Lys Homo sapiens complement component C2a + complement component C2b
-
?
complement component C4 + H2O cleavage of a single Arg-Ala bond in the sequence Leu-Gln-Arg-Ala-Leu-Glu Homo sapiens complement component C4a + complement component C4b
-
?
additional information binding of C1 to certain viral activators occurs through both C1s and C1q Homo sapiens ?
-
?
N-benzyloxycarbonyl-Lys-p-nitrophenyl ester + H2O N-alpha-benzyloxycarbonyl-L-Lys p-nitrophenyl ester Homo sapiens ?
-
?
N-benzyloxycarbonyl-Tyr-p-nitrophenyl ester + H2O
-
Homo sapiens ?
-
?
Nalpha-acetyl-Tyr ethyl ester + H2O
-
Homo sapiens ?
-
?
Nalpha-tosyl-Arg methyl ester + H2O
-
Homo sapiens ?
-
?
Tosyl-Arg methyl ester + H2O
-
Homo sapiens ?
-
?

Subunits

Subunits Comment Organism
? x * 56000, A chain, + x * 27000, B chain, gel filtration under denaturing conditions Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
56
-
30 min, 50% loss of activity Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8.5 acetyl-Tyr ethyl ester Homo sapiens

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 9.5 4°C, irreversible inactivation below pH 5.0 and above pH 9.5, overnight exposure Homo sapiens