General Stability | Organism |
---|---|
stable at concentrations of 1-2 mg/ml | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
amidines | - |
Homo sapiens | |
C1bar inhibitor | - |
Homo sapiens | |
DFP | - |
Homo sapiens | |
Guanidines | - |
Homo sapiens | |
p-nitrophenyl-p'-guanidinobenzoate | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.056 | 0.07 | N-benzyloxycarbonyl-Tyr p-nitrophenyl ester | - |
Homo sapiens | |
0.8 | - |
N-benzyloxycarbonyl-Lys p-nitrophenyl ester | - |
Homo sapiens | |
1.7 | 2.8 | tosyl-Arg methyl ester | - |
Homo sapiens | |
2.5 | 5 | benzoyl-Arg ethyl ester | - |
Homo sapiens | |
19 | - |
acetyl-L-Tyr ethyl ester | - |
Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27000 | - |
x * 56000, A chain, + x * 27000, B chain, gel filtration under denaturing conditions | Homo sapiens |
56000 | - |
x * 56000, A chain, + x * 27000, B chain, gel filtration under denaturing conditions | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | binding of C1 to certain viral activators occurs through both C1s and C1q | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | C1rbar cleaves a single Arg-Ile or Lys-Ile bond in proenzyme C1s | Homo sapiens |
proteolytic modification | C1s is the proenzyme of C1sbar | Homo sapiens |
side-chain modification | C1sbar A chain contains 6.5% carbohydrate per weight, C1sbar B chain contains 7.5% carbohydrate per weight | Homo sapiens |
Purification (Comment) | Organism |
---|---|
C1s and C1sbar | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Benzoyl-Arg ethyl ester + H2O | - |
Homo sapiens | ? | - |
? | |
complement component C2 + H2O | cleavage of a single bond, likely to be Lys-Lys or Arg-Lys | Homo sapiens | complement component C2a + complement component C2b | - |
? | |
complement component C4 + H2O | cleavage of a single Arg-Ala bond in the sequence Leu-Gln-Arg-Ala-Leu-Glu | Homo sapiens | complement component C4a + complement component C4b | - |
? | |
additional information | binding of C1 to certain viral activators occurs through both C1s and C1q | Homo sapiens | ? | - |
? | |
N-benzyloxycarbonyl-Lys-p-nitrophenyl ester + H2O | N-alpha-benzyloxycarbonyl-L-Lys p-nitrophenyl ester | Homo sapiens | ? | - |
? | |
N-benzyloxycarbonyl-Tyr-p-nitrophenyl ester + H2O | - |
Homo sapiens | ? | - |
? | |
Nalpha-acetyl-Tyr ethyl ester + H2O | - |
Homo sapiens | ? | - |
? | |
Nalpha-tosyl-Arg methyl ester + H2O | - |
Homo sapiens | ? | - |
? | |
Tosyl-Arg methyl ester + H2O | - |
Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 56000, A chain, + x * 27000, B chain, gel filtration under denaturing conditions | Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
56 | - |
30 min, 50% loss of activity | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8.5 | acetyl-Tyr ethyl ester | Homo sapiens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 9.5 | 4°C, irreversible inactivation below pH 5.0 and above pH 9.5, overnight exposure | Homo sapiens |