Application | Comment | Organism |
---|---|---|
pharmacology | inhibition of the complement pathway is of great therapeutic interest. The C1 complex is a very attractive target for selective inhibition of the classical complement pathway because it is the only member of the classical pathway that does not participate in the other complement pathways | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
recombinant expression of N-terminally His6-tagged enzyme fragment comprising the second CCP domain and the serine protease domain, CCP2-SP, amino acids 358-688 | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | engineering of a potenthuman antibody inhibitor of C1s protease activity, inhibition of recombinnat enzyme fragment CCP2-SP, overview. The C1 complex is a very attractive target for selective inhibition of the classical complement pathway because it is the only member of the classical pathway that does not participate in the other complement pathways | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Homo sapiens | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
complement C4 zymogen + H2O | Homo sapiens | - |
active complement C4 + ? | - |
? | |
complement C4-bound C2 zymogen + H2O | Homo sapiens | - |
C4a + C2b | the fragments associate to form a C3 convertase enzyme | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P09871 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | activation of protease C1s through cleavage by protease C1r, EC 3.4.21.41 | Homo sapiens |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme fragment CCP2 and SP by nickel affinity chromatography and dialysis, activation by C1r | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
blood serum | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
complement C4 zymogen + H2O | - |
Homo sapiens | active complement C4 + ? | - |
? | |
complement C4-bound C2 zymogen + H2O | - |
Homo sapiens | C4a + C2b | the fragments associate to form a C3 convertase enzyme | ? |
Synonyms | Comment | Organism |
---|---|---|
C1s protease | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
metabolism | In the classical complement pathway, immune complexes formed by IgM, IgG1, IgG2 and IgG3 antibodies result in the activation of the C1s protease that in turn cleaves C4 and then C4-bound-C2 yielding the proteolytic fragments C4b and C2a which associate to form a C3 convertase enzyme | Homo sapiens |