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Literature summary for 3.4.21.42 extracted from

  • Rossi, V.; Teillet, F.; Thielens, N.M.; Bally, I.; Arlaud, G.J.
    Functional characterization of complement proteases C1s/mannan-binding lectin-associated serine protease-2 (MASP-2) chimeras reveals the higher C4 recognition efficacy of the MASP-2 complement control protein modules (2005), J. Biol. Chem., 280, 41811-41818.
    View publication on PubMed
Search for more literature for 3.4.21.42

Cloned(Commentary)

Cloned (Comment) Organism
expression in a baculovirus/insect cells system Homo sapiens

Protein Variants

Protein Variants Comment Organism
C1s(MASP-2 CCP1/2) chimeric C1s protease containing the MASP-2 complement control protein domain, cleavage efficiency of C4 similar to MASP-2 protease Homo sapiens
C1s(MASP-2 SP) chimeric C1s protease containing the MASP-2 serine protease domain, cleavage efficiency similar to wild-type C1s Homo sapiens
D275S/P276I/K405V similar to wild-type C1s Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000085
-
 Complement component C4 145 mM NaCl, 50 mM triethanolamine hydrochloride, 1 mM CaCl2, 1 mg/ml OVA pH 7.4, 37°C Homo sapiens
0.0052
-
 Complement component C2 145 mM NaCl, 50 mM triethanolamine hydrochloride, 1 mM CaCl2, 1 mg/ml OVA pH 7.4, 37°C Homo sapiens
0.0061
-
 Complement component C2 145 mM NaCl, 50 mM triethanolamine hydrochloride, 1 mM CaCl2, 1 mg/ml ovalbumin pH 7.4, 37°C Homo sapiens
0.0066
-
 Complement component C4 145 mM NaCl, 50 mM triethanolamine hydrochloride, 1 mM CaCl2, 1 mg/ml ovalbumin pH 7.4, 37°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
one-step affinity chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
culture supernatant
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
complement component C2 + H2O
-
 Homo sapiens complement component C2a + complement component C2b
-
 ?
complement component C4 + H2O
-
 Homo sapiens complement component C4a + complement component C4b
-
 ?

Synonyms

Synonyms Comment Organism
C1s
-
 Homo sapiens
complement protease C1s
-
 Homo sapiens
mannan-binding lectin-associated serine protease-2
-
 Homo sapiens
MASP-2
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.9
-
 Complement component C4 145 mM NaCl, 50 mM triethanolamine hydrochloride, 1 mM CaCl2, 1 mg/ml OVA pH 7.4, 37°C Homo sapiens
5.1
-
 Complement component C2 145 mM NaCl, 50 mM triethanolamine hydrochloride, 1 mM CaCl2, 1 mg/ml ovalbumin pH 7.4, 37°C Homo sapiens
5.4
-
 Complement component C4 145 mM NaCl, 50 mM triethanolamine hydrochloride, 1 mM CaCl2, 1 mg/ml ovalbumin pH 7.4, 37°C Homo sapiens
5.6
-
 Complement component C2 145 mM NaCl, 50 mM triethanolamine hydrochloride, 1 mM CaCl2, 1 mg/ml OVA pH 7.4, 37°C Homo sapiens