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Literature summary for 3.4.21.41 extracted from
- Human complement serine proteases C1r and C1s and their proenzymes (1993), Methods Enzymol., 223, 61-82.
General Stability
| General Stability | Organism |
|---|---|
| C1rbar undergoes autolytic cleavage | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-Nitrophenyl-4-guanidinobenzoate | - |
Homo sapiens |  |
| Ca2+ | reaction with C1s | Homo sapiens | |
| diisopropyl fluorophosphate | - |
Homo sapiens | |
| leupeptin | competitive | Homo sapiens | |
| additional information | high ionic strength inhibits | Homo sapiens | |
| phenylmethylsulfonyl fluoride | - |
Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
MW of C1r: 166000-188000 Da, gel filtration | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| proteolytic modification | autolytic proteolysis involves cleavage of the Arg279-Gly280 bond in the sequence Asp-Ser-Arg-Gly-Trp-Lys | Homo sapiens |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| serum | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| complement component C1s + H2O | cleavage of a single Arg-Ile bond in the sequence Lys-Gln-Arg-Ile-Ile-Gly | Homo sapiens | complement component C1sbar | - |
? | |
| N-acetyl-Gly-Lys methyl ester + H2O | - |
Homo sapiens | N-acetyl-Gly-Lys + methanol | - |
? | |
| N-benzyloxycarbonyl-Gly-Arg thiobenzyl ester + H2O | - |
Homo sapiens | ? | - |
? | |
| N-carbobenzoxy-Lys-p-nitrophenyl ester + H2O | - |
Homo sapiens | N-carbobenzoxy-Lys + 4-nitrophenyl | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | C1rbar and the proenzyme C1r are noncovalent dimers, the subunit of C1r has a MW of 53000-85000 Da, SDS-PAGE | Homo sapiens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
high temperature transition occurs at 53°C, the low-temperature transition varies between 26°C at low ionic strength and 40°C in the presence of 0.5 M NaCl | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Homo sapiens |
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