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Literature summary for 3.4.21.41 extracted from

  • Kardos, J.; Gal, P.; Szilagyi, L.; Thielens, N.M.; Szilagyi, K.; Lorincz, Z.; Kulcsar, P.; Graf, L.; Arlaud, G.J.; Zavodszky, P.
    The role of the individual domains in the structure and function of the catalytic region of a modular serine protease, C1r (2001), J. Immunol., 167, 5202-5208.
    View publication on PubMed
Search for more literature for 3.4.21.41

Cloned(Commentary)

Cloned (Comment) Organism
production of the CCP1-CCP2-SP, the CCP2-SP and the SP fragments in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
S654A mutant without autoactivation Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information KM-values of the C1r fragments with benzyloxycarbonyl-Lys-thiobenzyl ester or benzyloxycarbonyl-Gly-Arg-thiobenzyl ester as substrate Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
 molecular weight of the CCP1-CCP2-SP fragments Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyloxycarbonyl-Gly-Arg-thiobenzyl ester + H2O
-
 Homo sapiens ?
-
 ?
benzyloxycarbonyl-Lys-thiobenzyl ester + H2O
-
 Homo sapiens benzyloxycarbonyl-Lys + phenylmethanethiol
-
 ?
complement component C1s + H2O
-
 Homo sapiens complement component C1sbar
-
 ?
additional information the ability of autoactivation of C1r and C1s cleavage activity is an inherent property of the SP domain Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
dimer deletion of CCP1 domain from CCP1-CCP2-SP fragment results in the loss of the dimeric structure. Dimerization of C1r is not a prerequisite for autoactivation Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 melting transition of SP fragment is 47.5°C, melting point of the CCP2-SP fragment is 55.4°C, the CCP1-CCP2-SP fragment shows an unfolding transition at 57.5°C Homo sapiens