Cloned (Comment) | Organism |
---|---|
baculovirus-mediated expression is used to produce fragments containing the SP domain and either 2 CCP modules (CCP1/2-SP) or only the second CCP module (CCP2-SP). In each case the wild-type species and two mutants stabilized in the proenzyme form by mutations at the cleavage site (R446Q) or at the active site serine residue (S637A) are produced | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
R446Q | stabilized in the single-chain proenzyme form by mutation at the cleavage site, no esterolytic activity with acetyl-Gly-Lys-methyl ester | Homo sapiens |
S637A | stabilized in the single-chain proenzyme form by mutation at the active site serine residue, no esterolytic activity with acetyl-Gly-Lys-methyl ester | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | activated C1r and its wild-type CCP1/2-SP and CCP2-SP fragments all cleave acetyl-Gly-Lys-methyl ester with KM-values ranging from 1.3 mM to 2.1 mM | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
molecular weight of C1r fragments | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | carbohydrate content of C1r fragments | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-Gly-Lys-methyl ester + H2O | - |
Homo sapiens | ? | - |
? | |
complement component C1s + H2O | C1R and its activated fragments all cleave C1s, in the order of increasing efficiency: C21r, CCP1/2-SP, CCP2-SP. CCP1 is not involved in C1s recognition | Homo sapiens | complement component C1sbar | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | CCP1 is essential to the assembly of the dimer, but formation of a stable dimer is not a prerequisite for self-activation | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | activated C1r and its wild-type CCP1/2-SP and CCP2-SP fragments all cleave acetyl-Gly-Lys-methyl ester with turnover numbers ranging from 8.2 s to 9.2 s | Homo sapiens |