Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.21.4 extracted from

  • Compton, P.D.; Coll, R.J.; Fink, A.L.
    Effects of methanol cryosolvents on the structural and catalytic properties of bovine trypsin (1986), J. Biol. Chem., 261, 1248-1252.
    View publication on PubMed

General Stability

General Stability Organism
stable in 70% methanol at 0°C and below, pH 6.4 Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
benzamidine
-
Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0012
-
Nalpha-benzyloxycarbonyl-Lys-p-nitrophenyl ester without addition of methanol Bos taurus
0.0038
-
Nalpha-benzyloxycarbonyl-Lys-p-nitrophenyl ester in presence of 10% methanol Bos taurus
0.0096
-
Nalpha-benzyloxycarbonyl-Lys-p-nitrophenyl ester in presence of 20% methanol Bos taurus
0.0176
-
Nalpha-benzyloxycarbonyl-Lys-p-nitrophenyl ester in presence of 30% methanol Bos taurus
0.168
-
Nalpha-benzyloxycarbonyl-Lys-p-nitrophenyl ester in presence of 50% methanol Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyloxycarbonyl-Lys p-nitrophenyl ester + H2O
-
Bos taurus benzyloxycarbonyl-Lys + p-nitrophenol
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.013
-
Nalpha-benzyloxycarbonyl-Lys-p-nitrophenyl ester without addition of methanol Bos taurus
0.043
-
Nalpha-benzyloxycarbonyl-Lys-p-nitrophenyl ester in presence of 10% methanol Bos taurus
0.052
-
Nalpha-benzyloxycarbonyl-Lys-p-nitrophenyl ester in presence of 20% methanol Bos taurus
0.07
-
Nalpha-benzyloxycarbonyl-Lys-p-nitrophenyl ester in presence of 30% methanol Bos taurus
0.13
-
Nalpha-benzyloxycarbonyl-Lys-p-nitrophenyl ester in presence of 50% methanol Bos taurus