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Literature summary for 3.4.21.4 extracted from
- Purification and characterization of trypsin from the poikilotherm Gadus morhua (1989), Eur. J. Biochem., 180, 85-94.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ovomucoid | - |
Gadus morhua | |
| p-tosyl-L-Lys-chloromethane | - |
Gadus morhua |  |
| phenylmethyl sulfonyl fluoride | - |
Gadus morhua | |
| Soybean trypsin inhibitor | - |
Gadus morhua |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.029 | - |
p-tosyl-L-Arg methyl ester | - |
Gadus morhua | |
| 0.077 | - |
Nalpha-benzoyl-DL-Arg-p-nitroanilide | - |
Gadus morhua | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 23200 | - |
x * 23200, SDS-PAGE | Gadus morhua |
| 24700 | - |
gel filtration | Gadus morhua |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gadus morhua | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Gadus morhua |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cecum | - |
Gadus morhua | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 142.8 | - |
enzyme II | Gadus morhua |
| 155.7 | - |
enzyme III | Gadus morhua |
| 339 | - |
enzyme I | Gadus morhua |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Nalpha-benzoyl-DL-Arg-p-nitroanilide + H2O | Nalpha-benzoyl-L-Arg-p-nitroanilide | Gadus morhua | ? | - |
? | |
| p-tosyl-L-Arg methyl ester + H2O | - |
Gadus morhua | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 23200, SDS-PAGE | Gadus morhua |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Gadus morhua |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 8.5 | pH 6.5: about 50% of maximal activity, pH 8.5: about 80% of maximal activity | Gadus morhua |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 2 | - |
30 min, 5°C, about 55% loss of maximal activity | Gadus morhua |
| 4 | - |
30 min, 5°C, about 15% loss of maximal activity | Gadus morhua |
| 5 | 7 | 5°C, highest stability | Gadus morhua |
| 10 | - |
30 min, 5°C, 15% loss of activity | Gadus morhua |
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