Literature summary for 3.4.21.4 extracted from

Wahlgren, W.Y.; Pal, G.; Kardos, J.; Porrogi, P.; Szenthe, B.; Patthy, A.; Graf, L.; Katona, G.
The catalytic aspartate is protonated in the Michaelis complex formed between trypsin and an in vitro evolved substrate-like inhibitor: a refined mechanism of serine protease action (2011), J. Biol. Chem., 286, 3587-3596.

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified enzyme in complex with T5E/N18R/T20G/P21S/T22D/K31M mutant Schistocerca gregaria protease (trypsin) inhibitor 1, hanging drop vapor diffusion method, mixing of protein solution, containing 9.1 mg/ml protein complex in 0.5 mM MES, pH 6.0, and precipitant solution, containing 30% PEG 4000, 0.3 M ammonium acetate, 0.1 M sodium acetate, pH 4.6, in a 1:2.5 molar ratio, equilibration against precipitation solution, X-ray diffraction structure determination and analysis at 0.93 A resolution. modeling	Bos taurus

Crystallization (Comment)

Organism

purified enzyme in complex with T5E/N18R/T20G/P21S/T22D/K31M mutant Schistocerca gregaria protease (trypsin) inhibitor 1, hanging drop vapor diffusion method, mixing of protein solution, containing 9.1 mg/ml protein complex in 0.5 mM MES, pH 6.0, and precipitant solution, containing 30% PEG 4000, 0.3 M ammonium acetate, 0.1 M sodium acetate, pH 4.6, in a 1:2.5 molar ratio, equilibration against precipitation solution, X-ray diffraction structure determination and analysis at 0.93 A resolution. modeling

Bos taurus

Inhibitors

Inhibitors	Comment	Organism	Structure
T5E/N18R/T20G/P21S/T22D/K31M mutant Schistocerca gregaria protease (trypsin) inhibitor 1	the mutant inhibitor is improved compared to the wild-type inhibitor protein	Bos taurus

Organism

Organism	UniProt	Comment	Textmining
Bos taurus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
further purification of the commercial preparation by gel filtration	Bos taurus

Reaction

Reaction	Comment	Organism	Reaction ID
preferential cleavage: Arg-/-, Lys-/-	catalytic mechanism involving both His57 and Asp102, overview	Bos taurus	a

Source Tissue

Source Tissue	Comment	Organism	Textmining
commercial preparation	-	Bos taurus	-

General Information

General Information	Comment	Organism
additional information	detailed geometry of the active site and the protonation state of catalytic residues, quantum chemical interpretation, overview	Bos taurus