Activating Compound | Comment | Organism | Structure |
---|---|---|---|
enterokinase | trypsinogens (0.0002 mM) are incubated at room temperature with 6-200 ng enterokinase in 0.1 M Tris (pH 8), containing 5 mM CaCl2 and 0.01% bovine serum albumin for 10-60 min | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
inactive trypsinogen-4 is expressed in Escherichia coli BL21(DE3) cells | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Aprotinin | very efficient inhibition of trypsin-1 and trypsin-2, but not of trypsin-4 | Homo sapiens | |
nexin-1 | nexin-1 inhibits isozymes trypsin-1 ad trypsin-2, nexin-1 inhibits trypsin-4 and forms stable complexes only with this trypsin isoenzyme | Homo sapiens | |
Pefabloc SC | completely inhibits isoenzymes trypsin-1, trypsin-2, and trypsin-4 | Homo sapiens | |
tumor-associated trypsin inhibitor | very efficient inhibition of trypsin-1 and trypsin-2, but not of trypsin-4 | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA Sepharose column chromatography and Resource S column chromatography | Homo sapiens |
Renatured (Comment) | Organism |
---|---|
denatured trypsinogen is rapidly diluted with re-folding buffer (0.7 M guanidine-HCl, 0.1 M Tris-HCl, pH 8.0, containing 10 mM benzamidine, 2 mM L-cysteine, and 1 mM L-cystine) | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | trypsinogen-4 is the only trypsin-isoenzyme expressed in the brain | Homo sapiens | - |
pancreas | isozymes trypsin-1 and trypsin-2 | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-benzoyl-L-isoleucyl-L-glutamyl-glycyl-L-arginine methyl ester + H2O | - |
Homo sapiens | N-benzoyl-L-isoleucyl-L-glutamyl-glycyl-L-arginine + methanol | - |
? | |
N-benzoyl-L-isoleucyl-L-glutamyl-glycyl-L-arginine-4-nitroanilide + H2O | - |
Homo sapiens | N-benzoyl-L-isoleucyl-L-glutamyl-glycyl-L-arginine + 4-nitroaniline | - |
? |
Synonyms | Comment | Organism |
---|---|---|
trypsin-1 | isozyme | Homo sapiens |
trypsin-2 | isozyme | Homo sapiens |
trypsin-4 | brain isozyme | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
23.5 | - |
N-benzoyl-L-isoleucyl-L-glutamyl-glycyl-L-arginine-4-nitroanilide | isozyme trypsin-1, in 50 mM Tris, pH 8.0, 100 mM NaCl, 6 mM CaCl2 | Homo sapiens | |
32.7 | - |
N-benzoyl-L-isoleucyl-L-glutamyl-glycyl-L-arginine-4-nitroanilide | isozyme trypsin-2, in 50 mM Tris, pH 8.0, 100 mM NaCl, 6 mM CaCl2 | Homo sapiens | |
70.5 | - |
N-benzoyl-L-isoleucyl-L-glutamyl-glycyl-L-arginine-4-nitroanilide | isozyme trypsin-4, in 50 mM Tris, pH 8.0, 100 mM NaCl, 6 mM CaCl2 | Homo sapiens |