Protein Variants | Comment | Organism |
---|---|---|
R193A | the mutation does not affect the activation energy of the reaction, but significantly alters the preexponential term of the Arrhenius equation | Homo sapiens |
R193F | the mutation does not affect the activation energy of the reaction, but significantly alters the preexponential term of the Arrhenius equation | Homo sapiens |
R193G | the mutation does not affect the activation energy of the reaction, but significantly alters the preexponential term of the Arrhenius equation | Homo sapiens |
R193Y | the mutation does not affect the activation energy of the reaction, but significantly alters the preexponential term of the Arrhenius equation | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0135 | - |
N-carbobenzyloxy-Gly-Pro-Arg-p-nitroanilide | mutant enzyme R193G, in 50 mM Tricine, 10 mM CaCl2, pH 8.0 at 20°C | Homo sapiens | |
0.0198 | - |
N-carbobenzyloxy-Gly-Pro-Arg-p-nitroanilide | mutant enzyme R193A, in 50 mM Tricine, 10 mM CaCl2, pH 8.0 at 20°C | Homo sapiens | |
0.0213 | - |
N-carbobenzyloxy-Gly-Pro-Arg-p-nitroanilide | mutant enzyme R193Y, in 50 mM Tricine, 10 mM CaCl2, pH 8.0 at 20°C | Homo sapiens | |
0.0252 | - |
N-carbobenzyloxy-Gly-Pro-Arg-p-nitroanilide | wild type enzyme, in 50 mM Tricine, 10 mM CaCl2, pH 8.0 at 20°C | Homo sapiens | |
0.0274 | - |
N-carbobenzyloxy-Gly-Pro-Arg-p-nitroanilide | mutant enzyme R193F, in 50 mM Tricine, 10 mM CaCl2, pH 8.0 at 20°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-carbobenzyloxy-Gly-Pro-Arg-p-nitroanilide + H2O | - |
Homo sapiens | N-carbobenzyloxy-Gly-Pro-Arg + p-nitroaniline | - |
? |
Synonyms | Comment | Organism |
---|---|---|
trypsin 4 | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
110 | - |
N-carbobenzyloxy-Gly-Pro-Arg-p-nitroanilide | mutant enzyme R193G, in 50 mM Tricine, 10 mM CaCl2, pH 8.0 at 20°C | Homo sapiens | |
124 | - |
N-carbobenzyloxy-Gly-Pro-Arg-p-nitroanilide | mutant enzyme R193A, in 50 mM Tricine, 10 mM CaCl2, pH 8.0 at 20°C | Homo sapiens | |
133 | - |
N-carbobenzyloxy-Gly-Pro-Arg-p-nitroanilide | mutant enzyme R193Y, in 50 mM Tricine, 10 mM CaCl2, pH 8.0 at 20°C | Homo sapiens | |
158 | - |
N-carbobenzyloxy-Gly-Pro-Arg-p-nitroanilide | wild type enzyme, in 50 mM Tricine, 10 mM CaCl2, pH 8.0 at 20°C | Homo sapiens | |
162 | - |
N-carbobenzyloxy-Gly-Pro-Arg-p-nitroanilide | mutant enzyme R193F, in 50 mM Tricine, 10 mM CaCl2, pH 8.0 at 20°C | Homo sapiens |