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Literature summary for 3.4.21.4 extracted from

  • Treetharnmathurot, B.; Ovartlarnporn, C.; Wungsintaweekul, J.; Duncan, R.; Wiwattanapatapee, R.
    Effect of PEG molecular weight and linking chemistry on the biological activity and thermal stability of PEGylated trypsin (2008), Int. J. Pharm., 357, 252-259.
    View publication on PubMed
Search for more literature for 3.4.21.4

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.88
-
 N-benzoyl-L-arginine-p-nitroanilide in 67 mM sodium phosphate buffer, pH 7.6, at 25°C Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein polyethyleneglycosylation Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
pancreas
-
 Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-alpha-benzoyl-L-arginine ethyl ester + H2O
-
 Bos taurus ?
-
 ?
N-benzoyl-L-arginine-p-nitroanilide + H2O
-
 Bos taurus N-benzoyl-L-arginine + p-nitroaniline
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 trypsin conjugated with mPEG 5000 having cyanurate linker demonstrates the best thermal stability at 70°C compared to the native enzyme Bos taurus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1
-
 N-benzoyl-L-arginine-p-nitroanilide in 67 mM sodium phosphate buffer, pH 7.6, at 25°C Bos taurus