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Literature summary for 3.4.21.4 extracted from
- Purification and characterization of trypsin from the spleen of Tongol tuna (Thunnus tonggol) (2006), J. Agric. Food Chem., 54, 5617-5622.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ethylenediaminetetraacetic acid | partial | Thunnus tonggol |  |
| N-p-tosyl-lysine chloroketone | - |
Thunnus tonggol | |
| Soybean trypsin inhibitor | strong | Thunnus tonggol |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.25 | - |
Nalpha-p-tosyl-L-Arg methyl ester | pH 8.0, 30°C | Thunnus tonggol | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | required for thermal stability | Thunnus tonggol | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 24000 | - |
gel filtration | Thunnus tonggol |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thunnus tonggol | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thunnus tonggol |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| spleen | - |
Thunnus tonggol | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 36.15 | - |
pH 8.0, 30°C | Thunnus tonggol |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Nalpha-p-tosyl-L-Arg methyl ester + H2O | - |
Thunnus tonggol | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 24000, SDS-PAGE | Thunnus tonggol |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
- |
Thunnus tonggol |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
unstable above, Ca2+ is required for thermal stability | Thunnus tonggol |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 200 | - |
Nalpha-p-tosyl-L-Arg methyl ester | pH 8.0, 30°C | Thunnus tonggol | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Thunnus tonggol |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 11 | - |
Thunnus tonggol |
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