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Literature summary for 3.4.21.4 extracted from

  • Ahsan, M.N.; Watabe, S.
    Kinetic and structural properties of two isoforms of trypsin isolated from the viscera of Japanese anchovy, Engraulis japonicus (2001), J. Protein Chem., 20, 49-58.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 0.1 mM, isoenzyme aT-I loses 90% of its activity, isoenzyme aT-II loses 93% of its activity Engraulis japonicus
antipain 0.01 mM, isoenzyme aT-I loses 96% of its activity, isoenzyme aT-II loses 99% of its activity Engraulis japonicus
benzamidine 1 mM, isoenzyme aT-I loses 90% of its activity, isoenzyme aT-II loses 93% of its activity Engraulis japonicus
leupeptin 0.1 mM, isoenzyme aT-I loses 97% of its activity, isoenzyme aT-II completely loses its activity Engraulis japonicus
PMSF 0.1 mM, isoenzyme aT-I loses 89% of its activity, isoenzyme aT-II loses 80% of its activity Engraulis japonicus
Soybean trypsin inhibitor 0.1 mg/ml, isoenzyme aT-I loses 95% of its activity, isoenzyme aT-II loses 97% of its activity Engraulis japonicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.014
-
benzoyl-Arg ethyl ester pH 8.0, 25°C, isoenzyme aT-II Engraulis japonicus
0.031
-
benzoyl-Arg ethyl ester pH 8.0, 25°C, isoenzyme aT-I Engraulis japonicus
0.033
-
benzoyl-Arg 4-nitroanilide pH 8.0, 25°C, isoenzyme aT-II Engraulis japonicus
0.037
-
benzoyl-Arg 4-nitroanilide pH 8.0, 25°C, isoenzyme aT-I Engraulis japonicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24000
-
isoenzyms aT-I and aT-II, gel filtration Engraulis japonicus

Organism

Organism UniProt Comment Textmining
Engraulis japonicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
two isoforms: aT-I and aT-II Engraulis japonicus

Source Tissue

Source Tissue Comment Organism Textmining
intestine visceral extract consisting mainly of pyloric ceca and intestine Engraulis japonicus
-
pyloric cecum visceral extract consisting mainly of pyloric ceca and intestine Engraulis japonicus
-
viscus consisting mainly of pyloric ceca and intestine Engraulis japonicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.85
-
aT-I Engraulis japonicus
9.26
-
aT-II Engraulis japonicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzoyl-Arg 4-nitroanilide + H2O
-
Engraulis japonicus benzoyl-Arg + 4-nitroaniline
-
?
benzoyl-Arg ethyl ester + H2O
-
Engraulis japonicus benzoyl-Arg + ethanol
-
?

Synonyms

Synonyms Comment Organism
aT-I
-
Engraulis japonicus
aT-II
-
Engraulis japonicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
isoform aT-I and aT-II Engraulis japonicus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 65 40°C: about 30% of maximal activity, 65°C: about 90% of maximal activity, isoenzyme aT-I. 40°C: about 30% of maximal activity, 65°C: about 70% of maximal activity, isoenzyme aT-II Engraulis japonicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
10
-
30 min, stable, isoenzyme aT-I and aT-II Engraulis japonicus
20
-
30 min, isoenzyme aT-I loses about 20% of its activity, isoenzyme aT-II loses about 10% of its activity Engraulis japonicus
30
-
30 min, isoenzyme aT-I loses about 25% of its activity, isoenzyme aT-II loses about 20% of its activity Engraulis japonicus
40
-
30 min, isoenzyme aT-I loses about 25% of its activity, isoenzyme aT-II loses about 20% of its activity Engraulis japonicus
50
-
30 min, isoenzyme aT-I loses about 25% of its activity, isoenzyme aT-II loses about 20% of its activity Engraulis japonicus
60
-
30 min, isoenzyme aT-I loses about 50% of its activity, isoenzyme aT-II loses about 55% of its activity Engraulis japonicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.3
-
benzoyl-Arg ethyl ester pH 8.0, 25°C, isoenzyme aT-II Engraulis japonicus
1.94
-
benzoyl-Arg 4-nitroanilide pH 8.0, 25°C, isoenzyme aT-I Engraulis japonicus
2.94
-
benzoyl-Arg 4-nitroanilide pH 8.0, 25°C, isoenzyme aT-I Engraulis japonicus
3.7
-
benzoyl-Arg 4-nitroanilide pH 8.0, 25°C, isoenzyme aT-II Engraulis japonicus
42.9
-
benzoyl-Arg ethyl ester pH 8.0, 25°C, isoenzyme aT-I Engraulis japonicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
isoform aT-I and aT-II Engraulis japonicus

pH Range

pH Minimum pH Maximum Comment Organism
7 12 pH 7.0: about 65% of maximal activity, pH 12: about 90% of maximal activity, isoenzyme aT-I. pH 7.0: about 75% of maximal activity, pH 12: about 80% of maximal activity, isoenzyme aT-II Engraulis japonicus