Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 0.1 mM, isoenzyme aT-I loses 90% of its activity, isoenzyme aT-II loses 93% of its activity | Engraulis japonicus | |
antipain | 0.01 mM, isoenzyme aT-I loses 96% of its activity, isoenzyme aT-II loses 99% of its activity | Engraulis japonicus | |
benzamidine | 1 mM, isoenzyme aT-I loses 90% of its activity, isoenzyme aT-II loses 93% of its activity | Engraulis japonicus | |
leupeptin | 0.1 mM, isoenzyme aT-I loses 97% of its activity, isoenzyme aT-II completely loses its activity | Engraulis japonicus | |
PMSF | 0.1 mM, isoenzyme aT-I loses 89% of its activity, isoenzyme aT-II loses 80% of its activity | Engraulis japonicus | |
Soybean trypsin inhibitor | 0.1 mg/ml, isoenzyme aT-I loses 95% of its activity, isoenzyme aT-II loses 97% of its activity | Engraulis japonicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.014 | - |
benzoyl-Arg ethyl ester | pH 8.0, 25°C, isoenzyme aT-II | Engraulis japonicus | |
0.031 | - |
benzoyl-Arg ethyl ester | pH 8.0, 25°C, isoenzyme aT-I | Engraulis japonicus | |
0.033 | - |
benzoyl-Arg 4-nitroanilide | pH 8.0, 25°C, isoenzyme aT-II | Engraulis japonicus | |
0.037 | - |
benzoyl-Arg 4-nitroanilide | pH 8.0, 25°C, isoenzyme aT-I | Engraulis japonicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24000 | - |
isoenzyms aT-I and aT-II, gel filtration | Engraulis japonicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Engraulis japonicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
two isoforms: aT-I and aT-II | Engraulis japonicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
intestine | visceral extract consisting mainly of pyloric ceca and intestine | Engraulis japonicus | - |
pyloric cecum | visceral extract consisting mainly of pyloric ceca and intestine | Engraulis japonicus | - |
viscus | consisting mainly of pyloric ceca and intestine | Engraulis japonicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.85 | - |
aT-I | Engraulis japonicus |
9.26 | - |
aT-II | Engraulis japonicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzoyl-Arg 4-nitroanilide + H2O | - |
Engraulis japonicus | benzoyl-Arg + 4-nitroaniline | - |
? | |
benzoyl-Arg ethyl ester + H2O | - |
Engraulis japonicus | benzoyl-Arg + ethanol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
aT-I | - |
Engraulis japonicus |
aT-II | - |
Engraulis japonicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
isoform aT-I and aT-II | Engraulis japonicus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 65 | 40°C: about 30% of maximal activity, 65°C: about 90% of maximal activity, isoenzyme aT-I. 40°C: about 30% of maximal activity, 65°C: about 70% of maximal activity, isoenzyme aT-II | Engraulis japonicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | - |
30 min, stable, isoenzyme aT-I and aT-II | Engraulis japonicus |
20 | - |
30 min, isoenzyme aT-I loses about 20% of its activity, isoenzyme aT-II loses about 10% of its activity | Engraulis japonicus |
30 | - |
30 min, isoenzyme aT-I loses about 25% of its activity, isoenzyme aT-II loses about 20% of its activity | Engraulis japonicus |
40 | - |
30 min, isoenzyme aT-I loses about 25% of its activity, isoenzyme aT-II loses about 20% of its activity | Engraulis japonicus |
50 | - |
30 min, isoenzyme aT-I loses about 25% of its activity, isoenzyme aT-II loses about 20% of its activity | Engraulis japonicus |
60 | - |
30 min, isoenzyme aT-I loses about 50% of its activity, isoenzyme aT-II loses about 55% of its activity | Engraulis japonicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
benzoyl-Arg ethyl ester | pH 8.0, 25°C, isoenzyme aT-II | Engraulis japonicus | |
1.94 | - |
benzoyl-Arg 4-nitroanilide | pH 8.0, 25°C, isoenzyme aT-I | Engraulis japonicus | |
2.94 | - |
benzoyl-Arg 4-nitroanilide | pH 8.0, 25°C, isoenzyme aT-I | Engraulis japonicus | |
3.7 | - |
benzoyl-Arg 4-nitroanilide | pH 8.0, 25°C, isoenzyme aT-II | Engraulis japonicus | |
42.9 | - |
benzoyl-Arg ethyl ester | pH 8.0, 25°C, isoenzyme aT-I | Engraulis japonicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
isoform aT-I and aT-II | Engraulis japonicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 12 | pH 7.0: about 65% of maximal activity, pH 12: about 90% of maximal activity, isoenzyme aT-I. pH 7.0: about 75% of maximal activity, pH 12: about 80% of maximal activity, isoenzyme aT-II | Engraulis japonicus |