Cloned (Comment) | Organism |
---|---|
enterokinase cleavage site and N-terminal His6-tag, expressed in HEK 293 EBNA cell | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
K192M | weakened preference for acidic amino acids at position P2' | Homo sapiens |
R143Q | weakened preference for acidic amino acids at position P2' | Homo sapiens |
R143Q/K192M | no preference for acidic amino acids at position P2' | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
x * 33000, SDS-PAGE (active enzyme) | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Homo sapiens |
Purification (Comment) | Organism |
---|---|
immobilized metal ion affinity chromatography (Ni2+), activation by His6-tag removal by cleavage with enterokinase, heparin-Sepharose chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mast cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methoxy-succinyl-Arg-Ala-Tyr-4-nitroanilide + H2O | - |
Homo sapiens | methoxy-succinyl-Arg-Ala-Tyr + 4-nitroaniline | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 33000, SDS-PAGE (active enzyme) | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
chymase | - |
Homo sapiens |