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Literature summary for 3.4.21.37 extracted from

  • Averhoff, P.; Kolbe, M.; Zychlinsky, A.; Weinrauch, Y.
    Single residue determines the specificity of neutrophil elastase for Shigella virulence factors (2008), J. Mol. Biol., 377, 1053-1066.
    View publication on PubMed
Search for more literature for 3.4.21.37

Cloned(Commentary)

Cloned (Comment) Organism
expressed in RBL-1 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
A213V the mutant shows increased kcat and Km values compared to the wild type enzyme Homo sapiens
F192A the mutant shows increased kcat and Km values compared to the wild type enzyme Homo sapiens
F192K the mutant shows increased kcat and Km values compared to the wild type enzyme Homo sapiens
F215A very low activity, the mutant shows decreased kcat and increased Km values compared to the wild type enzyme Homo sapiens
F215Y the mutant shows decreased kcat and Km values compared to the wild type enzyme Homo sapiens
N98T the mutant shows increased kcat and Km values compared to the wild type enzyme Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
N-methoxysuccinyl-Ala-Ala-Pro-Val chloromethyl ketone
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06
-
 N-methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide mutant enzyme F215Y, in 500 mM NaCl and 200 mM Tris-HCl, pH 8, at 37°C Homo sapiens
0.07
-
 N-methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide wild type enzyme, in 500 mM NaCl and 200 mM Tris-HCl, pH 8, at 37°C Homo sapiens
0.09
-
 N-methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide mutant enzyme N98T, in 500 mM NaCl and 200 mM Tris-HCl, pH 8, at 37°C Homo sapiens
0.79
-
 N-methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide mutant enzyme F192K, in 500 mM NaCl and 200 mM Tris-HCl, pH 8, at 37°C Homo sapiens
1.04
-
 N-methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide mutant enzyme F215A, in 500 mM NaCl and 200 mM Tris-HCl, pH 8, at 37°C Homo sapiens
1.17
-
 N-methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide mutant enzyme A213V, in 500 mM NaCl and 200 mM Tris-HCl, pH 8, at 37°C Homo sapiens
5.02
-
 N-methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide mutant enzyme F192A, in 500 mM NaCl and 200 mM Tris-HCl, pH 8, at 37°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
specific granule neutrophil granule Homo sapiens 42581
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
IpaB protein + H2O Homo sapiens neutrophil elastase cleaves IpaB protein after alanine, valine, isoleucine, or threonine residues ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
neutrophil
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
IpaB protein + H2O neutrophil elastase cleaves IpaB protein after alanine, valine, isoleucine, or threonine residues Homo sapiens ?
-
 ?
additional information does not cleave N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide Homo sapiens ?
-
 ?
N-methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide + H2O specific neutrophil elastase substrate Homo sapiens N-methoxysuccinyl-Ala-Ala-Pro-Val + 4-nitroaniline
-
 ?

Synonyms

Synonyms Comment Organism
neutrophil elastase
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.42
-
 N-methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide mutant enzyme F215A, in 500 mM NaCl and 200 mM Tris-HCl, pH 8, at 37°C Homo sapiens
5.48
-
 N-methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide mutant enzyme F215Y, in 500 mM NaCl and 200 mM Tris-HCl, pH 8, at 37°C Homo sapiens
6.71
-
 N-methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide wild type enzyme, in 500 mM NaCl and 200 mM Tris-HCl, pH 8, at 37°C Homo sapiens
8.05
-
 N-methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide mutant enzyme A213V, in 500 mM NaCl and 200 mM Tris-HCl, pH 8, at 37°C Homo sapiens
10.7
-
 N-methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide mutant enzyme F192A, in 500 mM NaCl and 200 mM Tris-HCl, pH 8, at 37°C Homo sapiens
11.63
-
 N-methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide mutant enzyme F192K, in 500 mM NaCl and 200 mM Tris-HCl, pH 8, at 37°C Homo sapiens
63.11
-
 N-methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide mutant enzyme N98T, in 500 mM NaCl and 200 mM Tris-HCl, pH 8, at 37°C Homo sapiens