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Literature summary for 3.4.21.37 extracted from

  • Redini, F.; Tixier, J.M.; Petitou, M.; Choay, J.; Robert, L.; Hornebeck, W.
    Inhibition of leucocyte elastase by heparin and its derivatives (1988), Biochem. J., 252, 515-519.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
heparin tight-binding non-competitive inhibitor, 90% inhibition at 0.5 ng/ml, higher chain length of oligosaccharides causes stronger inhibition, desulfated heparins lack inhibitory potential, ionic strength above 0.5 abolishes the inhibitory capacity of heparin Homo sapiens
heparin tight-binding non-competitive inhibitor, 90% inhibition at 0.5 ng/ml, higher chain length of oligosaccharides causes stronger inhibition, desulfated heparins lack inhibitory potential, ionic strength above 0.5 abolishes the inhibitory capacity of heparin Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5
-
succinyl-Ala-Ala-NH-4-nitroanilide pH 8.0, 37°C Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Elastin + H2O Homo sapiens involved in lung defense against bacterial infection, due to the ability of the enzyme to cleave cell surface proteins, body cells also might be damaged, involved in the onset of emphysema and rheumatoid arthritis ? protein cleaved at at least one location ?
Elastin + H2O Rattus norvegicus involved in lung defense against bacterial infection, due to the ability of the enzyme to cleave cell surface proteins, body cells might also be damaged, involved in the onset of emphysema and rheumatoid arthritis ? protein cleaved at at least one location ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
lung pleural exudates Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Elastin + H2O
-
Homo sapiens ?
-
?
Elastin + H2O
-
Rattus norvegicus ?
-
?
Elastin + H2O involved in lung defense against bacterial infection, due to the ability of the enzyme to cleave cell surface proteins, body cells also might be damaged, involved in the onset of emphysema and rheumatoid arthritis Homo sapiens ? protein cleaved at at least one location ?
Elastin + H2O involved in lung defense against bacterial infection, due to the ability of the enzyme to cleave cell surface proteins, body cells might also be damaged, involved in the onset of emphysema and rheumatoid arthritis Rattus norvegicus ? protein cleaved at at least one location ?
succinyl-Ala-Ala-NH-4-nitroanilide + H2O
-
Homo sapiens succinyl-Ala-Ala-NH2 + 4-nitroaniline
-
?
succinyl-Ala-Ala-NH-4-nitroanilide + H2O
-
Rattus norvegicus succinyl-Ala-Ala-NH2 + 4-nitroaniline
-
?

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00004
-
heparin 8 disaccharide units, pH 8.0, 37°C Rattus norvegicus
0.023
-
heparin 5 disaccharide units, pH 8.0, 37°C Rattus norvegicus
0.1
-
heparin 2 disaccharide units, pH 8.0, 37°C Rattus norvegicus